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Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease.

Carlsson, Malin; Shukla, Swati LU ; Petersson, Ann Cathrine; Segelmark, Mårten LU and Hellmark, Thomas LU (2011) In Journal of Cystic Fibrosis 10. p.265-271
Abstract
The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed... (More)
The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed with flow cytometry. We found that the ANCA associated strains in most cases showed pyocyanin negative phenotypes. These strains also induced less inflammatory response than the non-ANCA associated strains as shown by apoptosis and necrosis of epithelial cells and neutrophils. Our results suggest that colonization with strains of P. aeruginosa that induce a weak inflammatory response is associated with unfavorable outcome in CF. We speculate that inadequate control of pathogen proliferation through an insufficient inflammatory response results in a slowly increasing number of bacteria and accumulation of dying PMNs in the airways, contributing to progression in CF lung disease. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Cystic Fibrosis
volume
10
pages
265 - 271
publisher
Elsevier
external identifiers
  • wos:000292065900007
  • pmid:21463973
  • scopus:79958252832
ISSN
1873-5010
DOI
10.1016/j.jcf.2011.03.004
language
English
LU publication?
yes
id
e79554db-72c7-4a0c-a87d-0fa5d3e126b1 (old id 1937517)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21463973?dopt=Abstract
date added to LUP
2011-05-02 09:31:11
date last changed
2017-08-27 05:47:07
@article{e79554db-72c7-4a0c-a87d-0fa5d3e126b1,
  abstract     = {The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed with flow cytometry. We found that the ANCA associated strains in most cases showed pyocyanin negative phenotypes. These strains also induced less inflammatory response than the non-ANCA associated strains as shown by apoptosis and necrosis of epithelial cells and neutrophils. Our results suggest that colonization with strains of P. aeruginosa that induce a weak inflammatory response is associated with unfavorable outcome in CF. We speculate that inadequate control of pathogen proliferation through an insufficient inflammatory response results in a slowly increasing number of bacteria and accumulation of dying PMNs in the airways, contributing to progression in CF lung disease.},
  author       = {Carlsson, Malin and Shukla, Swati and Petersson, Ann Cathrine and Segelmark, Mårten and Hellmark, Thomas},
  issn         = {1873-5010},
  language     = {eng},
  pages        = {265--271},
  publisher    = {Elsevier},
  series       = {Journal of Cystic Fibrosis},
  title        = {Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease.},
  url          = {http://dx.doi.org/10.1016/j.jcf.2011.03.004},
  volume       = {10},
  year         = {2011},
}