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Towards a molecular phylogeny of the fungus gnat genus Boletina (Diptera: Mycetophilidae)

Martinsson, Svante; Kjaerandsen, Jostein LU and Sundberg, Per (2011) In Zoologica Scripta 40(3). p.272-281
Abstract
Boletina is a species rich genus of fungus gnats (Diptera: Mycetophilidae) with a mainly Holarctic distribution. The systematics within the genus has gained little attention and this is a first attempt to shed some light over the systematics of Boletina and to test the segregation of the genera Saigusaia and Aglaomyia from Boletina. The nuclear marker 28S and mitochondrial 16S, COI and CytB were amplified and sequenced for 23 taxa that were analysed separately and together with a broad sample of outgroup taxa obtained from GenBank, where also 18S sequences were added. Phylogenies were estimated using maximum likelihood, Bayesian inference and parsimony. We strengthen the hypothesized sister-group relationship between Docosia and Boletina,... (More)
Boletina is a species rich genus of fungus gnats (Diptera: Mycetophilidae) with a mainly Holarctic distribution. The systematics within the genus has gained little attention and this is a first attempt to shed some light over the systematics of Boletina and to test the segregation of the genera Saigusaia and Aglaomyia from Boletina. The nuclear marker 28S and mitochondrial 16S, COI and CytB were amplified and sequenced for 23 taxa that were analysed separately and together with a broad sample of outgroup taxa obtained from GenBank, where also 18S sequences were added. Phylogenies were estimated using maximum likelihood, Bayesian inference and parsimony. We strengthen the hypothesized sister-group relationship between Docosia and Boletina, but the genus Boletina as currently delimited appears to be paraphyletic and nested in a clade together with Aglaomyia, Coelosia and Gnoriste. The genus Saigusaia, on the other hand, seems to be well separated from Boletina. The Boletina erythropyga species group is consistently found as a distinct basal clade within Boletina s.l. The results obtained are otherwise ambiguous both for the taxa in focus and in some analyses globally with a statistically supported total breakdown of the traditional higher classification into tribes, subfamilies and even families. Interestingly, this breakdown almost disappeared when additional 18S sequences were added. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Zoologica Scripta
volume
40
issue
3
pages
272 - 281
publisher
Wiley-Blackwell
external identifiers
  • wos:000289529100004
  • scopus:79954587987
ISSN
0300-3256
DOI
10.1111/j.1463-6409.2011.00474.x
language
English
LU publication?
yes
id
0f5a42cc-8d1a-4aba-bedd-c681c8c55027 (old id 1965153)
date added to LUP
2011-05-23 11:05:23
date last changed
2017-11-19 03:56:44
@article{0f5a42cc-8d1a-4aba-bedd-c681c8c55027,
  abstract     = {Boletina is a species rich genus of fungus gnats (Diptera: Mycetophilidae) with a mainly Holarctic distribution. The systematics within the genus has gained little attention and this is a first attempt to shed some light over the systematics of Boletina and to test the segregation of the genera Saigusaia and Aglaomyia from Boletina. The nuclear marker 28S and mitochondrial 16S, COI and CytB were amplified and sequenced for 23 taxa that were analysed separately and together with a broad sample of outgroup taxa obtained from GenBank, where also 18S sequences were added. Phylogenies were estimated using maximum likelihood, Bayesian inference and parsimony. We strengthen the hypothesized sister-group relationship between Docosia and Boletina, but the genus Boletina as currently delimited appears to be paraphyletic and nested in a clade together with Aglaomyia, Coelosia and Gnoriste. The genus Saigusaia, on the other hand, seems to be well separated from Boletina. The Boletina erythropyga species group is consistently found as a distinct basal clade within Boletina s.l. The results obtained are otherwise ambiguous both for the taxa in focus and in some analyses globally with a statistically supported total breakdown of the traditional higher classification into tribes, subfamilies and even families. Interestingly, this breakdown almost disappeared when additional 18S sequences were added.},
  author       = {Martinsson, Svante and Kjaerandsen, Jostein and Sundberg, Per},
  issn         = {0300-3256},
  language     = {eng},
  number       = {3},
  pages        = {272--281},
  publisher    = {Wiley-Blackwell},
  series       = {Zoologica Scripta},
  title        = {Towards a molecular phylogeny of the fungus gnat genus Boletina (Diptera: Mycetophilidae)},
  url          = {http://dx.doi.org/10.1111/j.1463-6409.2011.00474.x},
  volume       = {40},
  year         = {2011},
}