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Gluconobacter oxydans DSM 50049 – an efficient biocatalyst for oxidation of 5-formyl-2-furancarboxylic acid (FFCA) to 2,5-furandicarboxylic acid (FDCA)

Sayed, Mahmoud LU orcid ; Ismail, Mohamed LU orcid ; Sivasubramanian, Anirudh ; Kawano, Riko ; Li, Chengsi ; Jonsdottir Glaser, Sara LU and Hatti-Kaul, Rajni LU (2025) In Microbial Cell Factories 24(1).
Abstract
2,5-Furandicarboxylic acid (FDCA) is a promising building block for biobased recyclable polymers and a platform for other potential biobased chemicals. The common route of its production is by oxidation of sugar-derived 5-hydroxymethylfurfural (HMF). Several reports on biocatalytic oxidation using whole microbial cells or enzymes have been reported, which offers potentially a greener alternative compared to the chemical process. HMF oxidases and aryl alcohol oxidases are the only enzymes able to catalyse the complete oxidation to FDCA, however at low concentrations and are subject to inhibition by the FFCA (5-formylfuran-2-carboxylic acid) intermediate. The present report presents a study on the oxidation of FFCA to FDCA using the... (More)
2,5-Furandicarboxylic acid (FDCA) is a promising building block for biobased recyclable polymers and a platform for other potential biobased chemicals. The common route of its production is by oxidation of sugar-derived 5-hydroxymethylfurfural (HMF). Several reports on biocatalytic oxidation using whole microbial cells or enzymes have been reported, which offers potentially a greener alternative compared to the chemical process. HMF oxidases and aryl alcohol oxidases are the only enzymes able to catalyse the complete oxidation to FDCA, however at low concentrations and are subject to inhibition by the FFCA (5-formylfuran-2-carboxylic acid) intermediate. The present report presents a study on the oxidation of FFCA to FDCA using the obligately aerobic bacterium Gluconobacter oxydans and identification of the enzymes catalyzing the reaction. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biotransformation, Coniferyl aldehyde dehydrogenase (CALDH), Furan Dicarboxylic acid (FDCA), Gluconobacter oxydans, Membrane-bound aldehyde dehydrogenase (MALDH), Whole cell biocatalyst
in
Microbial Cell Factories
volume
24
issue
1
article number
68
pages
13 pages
publisher
BioMed Central (BMC)
external identifiers
  • scopus:105000447740
  • pmid:40108655
ISSN
1475-2859
DOI
10.1186/s12934-025-02689-x
language
English
LU publication?
yes
id
19678c56-78fa-4749-aca0-239d8da39b53
date added to LUP
2025-03-21 13:27:50
date last changed
2025-04-21 07:44:33
@article{19678c56-78fa-4749-aca0-239d8da39b53,
  abstract     = {{2,5-Furandicarboxylic acid (FDCA) is a promising building block for biobased recyclable polymers and a platform for other potential biobased chemicals. The common route of its production is by oxidation of sugar-derived 5-hydroxymethylfurfural (HMF). Several reports on biocatalytic oxidation using whole microbial cells or enzymes have been reported, which offers potentially a greener alternative compared to the chemical process. HMF oxidases and aryl alcohol oxidases are the only enzymes able to catalyse the complete oxidation to FDCA, however at low concentrations and are subject to inhibition by the FFCA (5-formylfuran-2-carboxylic acid) intermediate. The present report presents a study on the oxidation of FFCA to FDCA using the obligately aerobic bacterium <i>Gluconobacter oxydans</i> and identification of the enzymes catalyzing the reaction.}},
  author       = {{Sayed, Mahmoud and Ismail, Mohamed and Sivasubramanian, Anirudh and Kawano, Riko and Li, Chengsi and Jonsdottir Glaser, Sara and Hatti-Kaul, Rajni}},
  issn         = {{1475-2859}},
  keywords     = {{Biotransformation; Coniferyl aldehyde dehydrogenase (CALDH); Furan Dicarboxylic acid (FDCA); Gluconobacter oxydans; Membrane-bound aldehyde dehydrogenase (MALDH); Whole cell biocatalyst}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{Microbial Cell Factories}},
  title        = {{<i>Gluconobacter oxydans</i> DSM 50049 – an efficient biocatalyst for oxidation of 5-formyl-2-furancarboxylic acid (FFCA) to 2,5-furandicarboxylic acid (FDCA)}},
  url          = {{http://dx.doi.org/10.1186/s12934-025-02689-x}},
  doi          = {{10.1186/s12934-025-02689-x}},
  volume       = {{24}},
  year         = {{2025}},
}