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Using 915 nm Laser Excited Tm3+/Er3+/Ho3+-Doped NaYbF4 Upconversion Nanoparticles for in Vitro and Deeper in Vivo Bioimaging without Overheating Irradiation

Zhan, Qiuqiang; Qian, Jun; Liang, Huijuan; Somesfalean, Gabriel LU ; Wang, Dan; He, Sailing; Zhang, Zhiguo and Andersson-Engels, Stefan LU (2011) In ACS Nano 5(5). p.3744-3757
Abstract
Successful further development of superhigh-constrast upconversion (UC) bioimaging requires addressing the existing paradox: 980 nm laser light is used to excite upconversion nanoparticles (UCNPs), while 980 nm light has strong optical absorption of water and biological specimens. The overheating caused by 980 nm excitation laser light in UC bioimaging is computationally and experimentally investigated for the first time. A new promising excitation approach for better near-infrared to near-infrared (NIR-to-NIR) UC photoluminescence in vitro or in vivo imaging is proposed employing a cost-effective 915 nm laser. This novel laser excitation method provides drastically less heating of the biological specimen and larger imaging depth In the... (More)
Successful further development of superhigh-constrast upconversion (UC) bioimaging requires addressing the existing paradox: 980 nm laser light is used to excite upconversion nanoparticles (UCNPs), while 980 nm light has strong optical absorption of water and biological specimens. The overheating caused by 980 nm excitation laser light in UC bioimaging is computationally and experimentally investigated for the first time. A new promising excitation approach for better near-infrared to near-infrared (NIR-to-NIR) UC photoluminescence in vitro or in vivo imaging is proposed employing a cost-effective 915 nm laser. This novel laser excitation method provides drastically less heating of the biological specimen and larger imaging depth In the animals or tissues due to quite low water absorption. Experimentally obtained thermal-graphic maps of the mouse in response to the laser heating are investigated to demonstrate the less heating advantage of the 915 nm laser. Our tissue phantom experiments and simulations verified that the 915 nm laser is superior to the 980 nm laser for deep tissue imaging. A novel and facile strategy for surface functionalization is utilized to render UCNPs hydrophilic, stable, and cell targeting. These as-prepared UCNPs were characterized by TEM, emission spectroscopy, XRD, FTIR, and zeta potential. Specifically targeting UCNPs excited with a 915 nm laser have shown very high contrast UC bioimaging. Highly stable DSPE-mPEG-5000-encapsulated UCNPs were injected into mice to perform in vivo imaging. Imaging and spectroscopy analysis of UC photoluminescence demonstrated that a 915 nm laser can serve as a new promising excitation light for UC animal imaging. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
upconversion nanoparticles, bioimaging, deep imaging, overheating free, near-infrared
in
ACS Nano
volume
5
issue
5
pages
3744 - 3757
publisher
The American Chemical Society
external identifiers
  • wos:000290826800040
  • scopus:80051529630
ISSN
1936-086X
DOI
10.1021/nn200110j
language
English
LU publication?
yes
id
ee73bbc1-d3f9-4c53-9276-e78a7973afad (old id 1986602)
date added to LUP
2011-06-29 11:09:43
date last changed
2017-10-22 03:27:19
@article{ee73bbc1-d3f9-4c53-9276-e78a7973afad,
  abstract     = {Successful further development of superhigh-constrast upconversion (UC) bioimaging requires addressing the existing paradox: 980 nm laser light is used to excite upconversion nanoparticles (UCNPs), while 980 nm light has strong optical absorption of water and biological specimens. The overheating caused by 980 nm excitation laser light in UC bioimaging is computationally and experimentally investigated for the first time. A new promising excitation approach for better near-infrared to near-infrared (NIR-to-NIR) UC photoluminescence in vitro or in vivo imaging is proposed employing a cost-effective 915 nm laser. This novel laser excitation method provides drastically less heating of the biological specimen and larger imaging depth In the animals or tissues due to quite low water absorption. Experimentally obtained thermal-graphic maps of the mouse in response to the laser heating are investigated to demonstrate the less heating advantage of the 915 nm laser. Our tissue phantom experiments and simulations verified that the 915 nm laser is superior to the 980 nm laser for deep tissue imaging. A novel and facile strategy for surface functionalization is utilized to render UCNPs hydrophilic, stable, and cell targeting. These as-prepared UCNPs were characterized by TEM, emission spectroscopy, XRD, FTIR, and zeta potential. Specifically targeting UCNPs excited with a 915 nm laser have shown very high contrast UC bioimaging. Highly stable DSPE-mPEG-5000-encapsulated UCNPs were injected into mice to perform in vivo imaging. Imaging and spectroscopy analysis of UC photoluminescence demonstrated that a 915 nm laser can serve as a new promising excitation light for UC animal imaging.},
  author       = {Zhan, Qiuqiang and Qian, Jun and Liang, Huijuan and Somesfalean, Gabriel and Wang, Dan and He, Sailing and Zhang, Zhiguo and Andersson-Engels, Stefan},
  issn         = {1936-086X},
  keyword      = {upconversion nanoparticles,bioimaging,deep imaging,overheating free,near-infrared},
  language     = {eng},
  number       = {5},
  pages        = {3744--3757},
  publisher    = {The American Chemical Society},
  series       = {ACS Nano},
  title        = {Using 915 nm Laser Excited Tm3+/Er3+/Ho3+-Doped NaYbF4 Upconversion Nanoparticles for in Vitro and Deeper in Vivo Bioimaging without Overheating Irradiation},
  url          = {http://dx.doi.org/10.1021/nn200110j},
  volume       = {5},
  year         = {2011},
}