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Isolation and Characterization of Shiga Toxin-Associated Microvesicles

Willysson, Annie LU ; Ståhl, Anne lie LU and Karpman, Diana LU orcid (2021) In Methods in Molecular Biology 2291. p.207-228
Abstract

Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.

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author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Cells, Electron microscopy, Enzyme-linked immunosorbent assay (ELISA), Extracellular vesicles, Flow cytometry, Live cell imaging, Microvesicles, Nanoparticle tracking analysis, Proteomics, Shiga toxin
host publication
Shiga Toxin-Producing E. coli
series title
Methods in Molecular Biology
volume
2291
pages
22 pages
publisher
Humana Press
external identifiers
  • pmid:33704755
  • scopus:85102845966
ISSN
1940-6029
1064-3745
ISBN
978-1-0716-1339-9
DOI
10.1007/978-1-0716-1339-9_9
language
English
LU publication?
yes
id
19c8e2f8-fef8-4b4f-bd98-321fefe6a3aa
date added to LUP
2021-03-31 12:40:37
date last changed
2025-10-14 13:03:40
@inbook{19c8e2f8-fef8-4b4f-bd98-321fefe6a3aa,
  abstract     = {{<p>Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.</p>}},
  author       = {{Willysson, Annie and Ståhl, Anne lie and Karpman, Diana}},
  booktitle    = {{Shiga Toxin-Producing E. coli}},
  isbn         = {{978-1-0716-1339-9}},
  issn         = {{1940-6029}},
  keywords     = {{Cells; Electron microscopy; Enzyme-linked immunosorbent assay (ELISA); Extracellular vesicles; Flow cytometry; Live cell imaging; Microvesicles; Nanoparticle tracking analysis; Proteomics; Shiga toxin}},
  language     = {{eng}},
  pages        = {{207--228}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Isolation and Characterization of Shiga Toxin-Associated Microvesicles}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-1339-9_9}},
  doi          = {{10.1007/978-1-0716-1339-9_9}},
  volume       = {{2291}},
  year         = {{2021}},
}