Isolation and Characterization of Shiga Toxin-Associated Microvesicles
(2021) In Methods in Molecular Biology 2291. p.207-228- Abstract
Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/19c8e2f8-fef8-4b4f-bd98-321fefe6a3aa
- author
- Willysson, Annie LU ; Ståhl, Anne lie LU and Karpman, Diana LU
- organization
- publishing date
- 2021
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Cells, Electron microscopy, Enzyme-linked immunosorbent assay (ELISA), Extracellular vesicles, Flow cytometry, Live cell imaging, Microvesicles, Nanoparticle tracking analysis, Proteomics, Shiga toxin
- host publication
- Shiga Toxin-Producing E. coli
- series title
- Methods in Molecular Biology
- volume
- 2291
- pages
- 22 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:33704755
- scopus:85102845966
- ISSN
- 1064-3745
- 1940-6029
- ISBN
- 978-1-0716-1339-9
- DOI
- 10.1007/978-1-0716-1339-9_9
- language
- English
- LU publication?
- yes
- id
- 19c8e2f8-fef8-4b4f-bd98-321fefe6a3aa
- date added to LUP
- 2021-03-31 12:40:37
- date last changed
- 2024-09-07 17:07:36
@inbook{19c8e2f8-fef8-4b4f-bd98-321fefe6a3aa, abstract = {{<p>Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.</p>}}, author = {{Willysson, Annie and Ståhl, Anne lie and Karpman, Diana}}, booktitle = {{Shiga Toxin-Producing E. coli}}, isbn = {{978-1-0716-1339-9}}, issn = {{1064-3745}}, keywords = {{Cells; Electron microscopy; Enzyme-linked immunosorbent assay (ELISA); Extracellular vesicles; Flow cytometry; Live cell imaging; Microvesicles; Nanoparticle tracking analysis; Proteomics; Shiga toxin}}, language = {{eng}}, pages = {{207--228}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Isolation and Characterization of Shiga Toxin-Associated Microvesicles}}, url = {{http://dx.doi.org/10.1007/978-1-0716-1339-9_9}}, doi = {{10.1007/978-1-0716-1339-9_9}}, volume = {{2291}}, year = {{2021}}, }