Evidence for a morphologically distinct and functionally robust cell type in the proximal tubules of human kidney.
(2014) In Human Pathology 45(2). p.382-393- Abstract
- Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller... (More)
- Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller and have drastically fewer mitochondria than the surrounding proximal tubule cells. They also display strong expression of several structural proteins such as vimentin, collagen-7A1 and the tight junction protein claudin-1. To functionally assess these cells, we also developed a novel human kidney explant model of ATN demonstrating that the cells are more resilient to injury than the surrounding proximal tubular cells. Taken together the results suggest a novel robust cell type with a contrasting biological role to that of the bulk of proximal tubular epithelium. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4291184
- author
- Hansson, Jennifer LU ; Hultenby, Kjell ; Cramnert, Catharina ; Pontén, Fredrik ; Jansson, Hannes LU ; Lindgren, David LU ; Axelson, Håkan LU and Johansson, Martin LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Human Pathology
- volume
- 45
- issue
- 2
- pages
- 382 - 393
- publisher
- Elsevier
- external identifiers
-
- pmid:24439225
- wos:000330551300024
- scopus:84892760007
- pmid:24439225
- ISSN
- 1532-8392
- DOI
- 10.1016/j.humpath.2013.10.003
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology (Malmö) (013031000), Oncology, MV (013035000), Connective Tissue Biology (013230151), Department of Translational Medicine (013017500)
- id
- 1a924845-5f32-43cf-8bdd-2eeae755a5b0 (old id 4291184)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24439225?dopt=Abstract
- date added to LUP
- 2016-04-01 09:49:06
- date last changed
- 2022-04-27 07:50:28
@article{1a924845-5f32-43cf-8bdd-2eeae755a5b0,
abstract = {{Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller and have drastically fewer mitochondria than the surrounding proximal tubule cells. They also display strong expression of several structural proteins such as vimentin, collagen-7A1 and the tight junction protein claudin-1. To functionally assess these cells, we also developed a novel human kidney explant model of ATN demonstrating that the cells are more resilient to injury than the surrounding proximal tubular cells. Taken together the results suggest a novel robust cell type with a contrasting biological role to that of the bulk of proximal tubular epithelium.}},
author = {{Hansson, Jennifer and Hultenby, Kjell and Cramnert, Catharina and Pontén, Fredrik and Jansson, Hannes and Lindgren, David and Axelson, Håkan and Johansson, Martin}},
issn = {{1532-8392}},
language = {{eng}},
number = {{2}},
pages = {{382--393}},
publisher = {{Elsevier}},
series = {{Human Pathology}},
title = {{Evidence for a morphologically distinct and functionally robust cell type in the proximal tubules of human kidney.}},
url = {{https://lup.lub.lu.se/search/files/1282446/4587164.pdf}},
doi = {{10.1016/j.humpath.2013.10.003}},
volume = {{45}},
year = {{2014}},
}