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Acoustic cell patterning in hydrogel for three-dimensional cell network formation

Koo, Kyo In LU orcid ; Lenshof, Andreas LU orcid ; Huong, Le Thi and Laurell, Thomas LU (2021) In Micromachines 12(1).
Abstract

In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The... (More)

In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acoustofluidics, Fibroblast cells, Three-dimensional network structure, Tissue engineering
in
Micromachines
volume
12
issue
1
article number
3
pages
12 pages
publisher
MDPI AG
external identifiers
  • scopus:85098700375
  • pmid:33375050
ISSN
2072-666X
DOI
10.3390/mi12010003
language
English
LU publication?
yes
id
1b01f49b-0e80-4b0a-88ea-a482a367b467
date added to LUP
2021-01-13 13:01:21
date last changed
2025-04-04 14:21:06
@article{1b01f49b-0e80-4b0a-88ea-a482a367b467,
  abstract     = {{<p>In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.</p>}},
  author       = {{Koo, Kyo In and Lenshof, Andreas and Huong, Le Thi and Laurell, Thomas}},
  issn         = {{2072-666X}},
  keywords     = {{Acoustofluidics; Fibroblast cells; Three-dimensional network structure; Tissue engineering}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Micromachines}},
  title        = {{Acoustic cell patterning in hydrogel for three-dimensional cell network formation}},
  url          = {{http://dx.doi.org/10.3390/mi12010003}},
  doi          = {{10.3390/mi12010003}},
  volume       = {{12}},
  year         = {{2021}},
}