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Acoustofluidic Properties of Polystyrene Microparticles

Edthofer, Alexander LU ; Novotny, Jakub LU orcid ; Lenshof, Andreas LU ; Laurell, Thomas LU and Baasch, Thierry LU (2023) In Analytical Chemistry 95(27). p.10346-10352
Abstract

Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is to measure the compressibility of cells and small organisms, which is related to their contents. The cell compressibility can be extracted from the acoustic mobility, which is the main output of acoustic migration experiments, if the material properties and sizes of reference particles, the size of the cells, and the surrounding medium are known. Accurate methods to measure and calibrate the acoustic energy density in acoustophoresis systems are therefore critical. In this Perspective, polystyrene... (More)

Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is to measure the compressibility of cells and small organisms, which is related to their contents. The cell compressibility can be extracted from the acoustic mobility, which is the main output of acoustic migration experiments, if the material properties and sizes of reference particles, the size of the cells, and the surrounding medium are known. Accurate methods to measure and calibrate the acoustic energy density in acoustophoresis systems are therefore critical. In this Perspective, polystyrene microparticles have become the most commonly used reference particles in acoustophoresis, due to their similar biophysical properties to cells. We utilized a two-step focusing method to measure the relative acoustic mobility of polystyrene beads of various sizes and colors and present a quantitative analysis of the variation in acousto-mechanical properties of polystyrene microparticles, showing a large spread in their material properties. A variation of more than 25% between different particle types was found. Thus, care is required when relying on polystyrene particles as a reference when characterizing acoustofluidics systems or acousto-mechanical properties of cells.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
95
issue
27
pages
10346 - 10352
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:37363950
  • scopus:85164422922
ISSN
0003-2700
DOI
10.1021/acs.analchem.3c01156
language
English
LU publication?
yes
id
1bdd6387-d33f-424a-8785-e13be448cca0
date added to LUP
2023-10-06 14:26:05
date last changed
2024-04-19 02:02:02
@article{1bdd6387-d33f-424a-8785-e13be448cca0,
  abstract     = {{<p>Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is to measure the compressibility of cells and small organisms, which is related to their contents. The cell compressibility can be extracted from the acoustic mobility, which is the main output of acoustic migration experiments, if the material properties and sizes of reference particles, the size of the cells, and the surrounding medium are known. Accurate methods to measure and calibrate the acoustic energy density in acoustophoresis systems are therefore critical. In this Perspective, polystyrene microparticles have become the most commonly used reference particles in acoustophoresis, due to their similar biophysical properties to cells. We utilized a two-step focusing method to measure the relative acoustic mobility of polystyrene beads of various sizes and colors and present a quantitative analysis of the variation in acousto-mechanical properties of polystyrene microparticles, showing a large spread in their material properties. A variation of more than 25% between different particle types was found. Thus, care is required when relying on polystyrene particles as a reference when characterizing acoustofluidics systems or acousto-mechanical properties of cells.</p>}},
  author       = {{Edthofer, Alexander and Novotny, Jakub and Lenshof, Andreas and Laurell, Thomas and Baasch, Thierry}},
  issn         = {{0003-2700}},
  language     = {{eng}},
  number       = {{27}},
  pages        = {{10346--10352}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Analytical Chemistry}},
  title        = {{Acoustofluidic Properties of Polystyrene Microparticles}},
  url          = {{http://dx.doi.org/10.1021/acs.analchem.3c01156}},
  doi          = {{10.1021/acs.analchem.3c01156}},
  volume       = {{95}},
  year         = {{2023}},
}