Acoustofluidic Properties of Polystyrene Microparticles
(2023) In Analytical Chemistry 95(27). p.10346-10352- Abstract
Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is to measure the compressibility of cells and small organisms, which is related to their contents. The cell compressibility can be extracted from the acoustic mobility, which is the main output of acoustic migration experiments, if the material properties and sizes of reference particles, the size of the cells, and the surrounding medium are known. Accurate methods to measure and calibrate the acoustic energy density in acoustophoresis systems are therefore critical. In this Perspective, polystyrene... (More)
Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is to measure the compressibility of cells and small organisms, which is related to their contents. The cell compressibility can be extracted from the acoustic mobility, which is the main output of acoustic migration experiments, if the material properties and sizes of reference particles, the size of the cells, and the surrounding medium are known. Accurate methods to measure and calibrate the acoustic energy density in acoustophoresis systems are therefore critical. In this Perspective, polystyrene microparticles have become the most commonly used reference particles in acoustophoresis, due to their similar biophysical properties to cells. We utilized a two-step focusing method to measure the relative acoustic mobility of polystyrene beads of various sizes and colors and present a quantitative analysis of the variation in acousto-mechanical properties of polystyrene microparticles, showing a large spread in their material properties. A variation of more than 25% between different particle types was found. Thus, care is required when relying on polystyrene particles as a reference when characterizing acoustofluidics systems or acousto-mechanical properties of cells.
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- author
- Edthofer, Alexander LU ; Novotny, Jakub LU ; Lenshof, Andreas LU ; Laurell, Thomas LU and Baasch, Thierry LU
- organization
-
- LTH Profile Area: Engineering Health
- Department of Biomedical Engineering
- MultiPark: Multidisciplinary research focused on ParkinsonĀ“s disease
- LU Profile Area: Light and Materials
- LTH Profile Area: Nanoscience and Semiconductor Technology
- LUCC: Lund University Cancer Centre
- Electrical Engineering (M.Sc.Eng.)
- Acoustofluidics group (research group)
- NanoLund: Centre for Nanoscience
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 95
- issue
- 27
- pages
- 10346 - 10352
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:37363950
- scopus:85164422922
- ISSN
- 0003-2700
- DOI
- 10.1021/acs.analchem.3c01156
- language
- English
- LU publication?
- yes
- id
- 1bdd6387-d33f-424a-8785-e13be448cca0
- date added to LUP
- 2023-10-06 14:26:05
- date last changed
- 2024-04-19 02:02:02
@article{1bdd6387-d33f-424a-8785-e13be448cca0, abstract = {{<p>Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is to measure the compressibility of cells and small organisms, which is related to their contents. The cell compressibility can be extracted from the acoustic mobility, which is the main output of acoustic migration experiments, if the material properties and sizes of reference particles, the size of the cells, and the surrounding medium are known. Accurate methods to measure and calibrate the acoustic energy density in acoustophoresis systems are therefore critical. In this Perspective, polystyrene microparticles have become the most commonly used reference particles in acoustophoresis, due to their similar biophysical properties to cells. We utilized a two-step focusing method to measure the relative acoustic mobility of polystyrene beads of various sizes and colors and present a quantitative analysis of the variation in acousto-mechanical properties of polystyrene microparticles, showing a large spread in their material properties. A variation of more than 25% between different particle types was found. Thus, care is required when relying on polystyrene particles as a reference when characterizing acoustofluidics systems or acousto-mechanical properties of cells.</p>}}, author = {{Edthofer, Alexander and Novotny, Jakub and Lenshof, Andreas and Laurell, Thomas and Baasch, Thierry}}, issn = {{0003-2700}}, language = {{eng}}, number = {{27}}, pages = {{10346--10352}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Acoustofluidic Properties of Polystyrene Microparticles}}, url = {{http://dx.doi.org/10.1021/acs.analchem.3c01156}}, doi = {{10.1021/acs.analchem.3c01156}}, volume = {{95}}, year = {{2023}}, }