Ucon-benzoyl dextran aqueous two-phase systems: protein purification with phase component recycling
(1996) In Journal of Chromatography. B 680(1-2). p.65-70- Abstract
- Benzoyl dextran with a degree of substitution of 0.18 was synthesized by reacting dextran T500 with benzoyl chloride. A new type of aqueous two-phase system composed of benzoyl dextran as bottom phase polymer and the random copolymer of ethylene oxide and propylene oxide (Ucon 50-HB-5100) as top phase polymer has been formed. The phase diagram for the system Ucon 50-HB-5100-benzoyl dextran with a degree of substitution of 0.18 was determined at room temperature. This two-phase system has been used to purify 3-phosphoglycerate kinase from baker's yeast. The top-phase polymer (Ucon) can be separated from target enzyme by increasing the temperature. The bottom-phase polymer (benzoyl dextran) could be recovered by addition of salt. Yeast... (More)
- Benzoyl dextran with a degree of substitution of 0.18 was synthesized by reacting dextran T500 with benzoyl chloride. A new type of aqueous two-phase system composed of benzoyl dextran as bottom phase polymer and the random copolymer of ethylene oxide and propylene oxide (Ucon 50-HB-5100) as top phase polymer has been formed. The phase diagram for the system Ucon 50-HB-5100-benzoyl dextran with a degree of substitution of 0.18 was determined at room temperature. This two-phase system has been used to purify 3-phosphoglycerate kinase from baker's yeast. The top-phase polymer (Ucon) can be separated from target enzyme by increasing the temperature. The bottom-phase polymer (benzoyl dextran) could be recovered by addition of salt. Yeast homogenate was partitioned in a primary Ucon 50-HB-5100-benzoyl dextran aqueous two-phase system. After phase separation the top phase was removed and temperature-induced phase separation was used for formation of a water phase and a Ucon-rich phase. The benzoyl dextran-enriched bottom phase from the primary system was diluted, and the polymer was separated from water by addition of Na2SO4. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126314
- author
- Lu, Min ; Albertsson, Per-Åke LU ; Johansson, Göte and Tjerneld, Folke LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aqueous two-phase system, Partitioning, Ucon, Benzoyl dextran, Protein
- in
- Journal of Chromatography. B
- volume
- 680
- issue
- 1-2
- pages
- 65 - 70
- publisher
- Elsevier
- external identifiers
-
- scopus:0029953740
- ISSN
- 1572-6495
- DOI
- 10.1016/0378-4347(95)00484-X
- language
- English
- LU publication?
- yes
- id
- 1c0f4888-3997-4bde-a2bf-8187fbb4ac1b (old id 126314)
- date added to LUP
- 2016-04-01 11:47:13
- date last changed
- 2022-01-26 18:13:28
@article{1c0f4888-3997-4bde-a2bf-8187fbb4ac1b,
abstract = {{Benzoyl dextran with a degree of substitution of 0.18 was synthesized by reacting dextran T500 with benzoyl chloride. A new type of aqueous two-phase system composed of benzoyl dextran as bottom phase polymer and the random copolymer of ethylene oxide and propylene oxide (Ucon 50-HB-5100) as top phase polymer has been formed. The phase diagram for the system Ucon 50-HB-5100-benzoyl dextran with a degree of substitution of 0.18 was determined at room temperature. This two-phase system has been used to purify 3-phosphoglycerate kinase from baker's yeast. The top-phase polymer (Ucon) can be separated from target enzyme by increasing the temperature. The bottom-phase polymer (benzoyl dextran) could be recovered by addition of salt. Yeast homogenate was partitioned in a primary Ucon 50-HB-5100-benzoyl dextran aqueous two-phase system. After phase separation the top phase was removed and temperature-induced phase separation was used for formation of a water phase and a Ucon-rich phase. The benzoyl dextran-enriched bottom phase from the primary system was diluted, and the polymer was separated from water by addition of Na2SO4.}},
author = {{Lu, Min and Albertsson, Per-Åke and Johansson, Göte and Tjerneld, Folke}},
issn = {{1572-6495}},
keywords = {{Aqueous two-phase system; Partitioning; Ucon; Benzoyl dextran; Protein}},
language = {{eng}},
number = {{1-2}},
pages = {{65--70}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography. B}},
title = {{Ucon-benzoyl dextran aqueous two-phase systems: protein purification with phase component recycling}},
url = {{http://dx.doi.org/10.1016/0378-4347(95)00484-X}},
doi = {{10.1016/0378-4347(95)00484-X}},
volume = {{680}},
year = {{1996}},
}