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Effects of Dextran-70 and Albumin on Coagulation in Experimental Hemorrhage in the Guinea Pig

Schött, Ulf LU ; Kander, Thomas LU and Bentzer, Peter LU (2017) In Shock
Abstract (Swedish)
Background: Dextran-70 is a more potent plasma volume expander than albumin but use has been
hampered because of it´s antithrombotic properties. However, also albumin has antithrombotic
properties and little is known about relative effects of these two colloids on coagulation in-vivo when
controlling for differences in efficacy as plasma volume expanders. Aim: Compare effects of dextran-
70 and albumin on coagulation at a dose resulting in equal plasma volume expansion. Methods:
Guinea pigs were subjected to a 25 ml/kg hemorrhage during 20 minutes and randomized to
resuscitation with either 6% dextran-70 at a dose of 15 ml/kg or 5% albumin at a dose of 25 ml/kg
(n=14 in each group) during 30 minutes starting one... (More)
Background: Dextran-70 is a more potent plasma volume expander than albumin but use has been
hampered because of it´s antithrombotic properties. However, also albumin has antithrombotic
properties and little is known about relative effects of these two colloids on coagulation in-vivo when
controlling for differences in efficacy as plasma volume expanders. Aim: Compare effects of dextran-
70 and albumin on coagulation at a dose resulting in equal plasma volume expansion. Methods:
Guinea pigs were subjected to a 25 ml/kg hemorrhage during 20 minutes and randomized to
resuscitation with either 6% dextran-70 at a dose of 15 ml/kg or 5% albumin at a dose of 25 ml/kg
(n=14 in each group) during 30 minutes starting one hour of shock. Blood samples were collected at
completion of resuscitation and at 4 hours. Plasma volume was measured using 125I-albumin and effect
on coagulation was evaluated using whole blood thrombelastography (TEG), measurement of
plasmafibrinogen and vonWillebrandfactor (vWF) concentrations and vWF GP1bA activity. Results:
Plasma volumes after resuscitation were similar in the groups at both time points. Dextran-70 resulted
in a transient prolongation of TEGclot amplification time (K) at completion of resuscitation compared
to albumin. TEG clot initiation (R) and strength (MA) did not differ between the treatments at any of
the time points. Albumin reduced vWF concentrations to a larger extent than dextran at both time
points whereas no difference in p-vWF GP1bA activity or in plasma fibrinogen and could be detected.
Conclusion: In equipotent doses with regard to plasma volume expansion, dextran-70 transiently
prolongsclot amplification time more than albumin whereas dextran-70 reducesplasma vWF
concentrations less than albumin. (Less)
Abstract

BACKGROUND: Dextran-70 is a more potent plasma volume expander than albumin but use has been hampered because of it's antithrombotic properties. However, also albumin has antithrombotic properties and little is known about relative effects of these two colloids on coagulation in-vivo when controlling for differences in efficacy as plasma volume expanders.

AIM: Compare effects of dextran-70 and albumin on coagulation at a dose resulting in equal plasma volume expansion.

METHODS: Guinea pigs were subjected to a 25 ml/kg hemorrhage during 20 minutes and randomized to resuscitation with either 6% dextran-70 at a dose of 15 ml/kg or 5% albumin at a dose of 25 ml/kg (n = 14 in each group) during 30 minutes starting one hour of... (More)

BACKGROUND: Dextran-70 is a more potent plasma volume expander than albumin but use has been hampered because of it's antithrombotic properties. However, also albumin has antithrombotic properties and little is known about relative effects of these two colloids on coagulation in-vivo when controlling for differences in efficacy as plasma volume expanders.

AIM: Compare effects of dextran-70 and albumin on coagulation at a dose resulting in equal plasma volume expansion.

METHODS: Guinea pigs were subjected to a 25 ml/kg hemorrhage during 20 minutes and randomized to resuscitation with either 6% dextran-70 at a dose of 15 ml/kg or 5% albumin at a dose of 25 ml/kg (n = 14 in each group) during 30 minutes starting one hour of shock. Blood samples were collected at completion of resuscitation and at 4 hours. Plasma volume was measured using I-albumin and effect on coagulation was evaluated using whole blood thrombelastography (TEG), measurement of plasma fibrinogen and von Willebrand factor (vWF) concentrations and vWF GP1bA activity.

RESULTS: Plasma volumes after resuscitation were similar in the groups at both time points. Dextran-70 resulted in a transient prolongation of TEG clot amplification time (K) at completion of resuscitation compared to albumin. TEG clot initiation (R) and strength (MA) did not differ between the treatments at any of the time points. Albumin reduced vWF concentrations to a larger extent than dextran at both time points whereas no difference in p-vWF GP1bA activity or in plasma fibrinogen and could be detected.

CONCLUSION: In equipotent doses with regard to plasma volume expansion, dextran-70 transiently prolongs clot amplification time more than albumin whereas dextran-70 reduces plasma vWF concentrations less than albumin.This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Shock
pages
15 pages
publisher
BioMedical Press
ISSN
1540-0514
DOI
10.1097/SHK.0000000000001025
language
English
LU publication?
yes
id
1e830326-3bcb-4599-87fb-0d36d25f0547
date added to LUP
2018-01-08 13:02:31
date last changed
2018-01-08 13:24:56
@article{1e830326-3bcb-4599-87fb-0d36d25f0547,
  abstract     = {<p>BACKGROUND: Dextran-70 is a more potent plasma volume expander than albumin but use has been hampered because of it's antithrombotic properties. However, also albumin has antithrombotic properties and little is known about relative effects of these two colloids on coagulation in-vivo when controlling for differences in efficacy as plasma volume expanders.</p><p>AIM: Compare effects of dextran-70 and albumin on coagulation at a dose resulting in equal plasma volume expansion.</p><p>METHODS: Guinea pigs were subjected to a 25 ml/kg hemorrhage during 20 minutes and randomized to resuscitation with either 6% dextran-70 at a dose of 15 ml/kg or 5% albumin at a dose of 25 ml/kg (n = 14 in each group) during 30 minutes starting one hour of shock. Blood samples were collected at completion of resuscitation and at 4 hours. Plasma volume was measured using I-albumin and effect on coagulation was evaluated using whole blood thrombelastography (TEG), measurement of plasma fibrinogen and von Willebrand factor (vWF) concentrations and vWF GP1bA activity.</p><p>RESULTS: Plasma volumes after resuscitation were similar in the groups at both time points. Dextran-70 resulted in a transient prolongation of TEG clot amplification time (K) at completion of resuscitation compared to albumin. TEG clot initiation (R) and strength (MA) did not differ between the treatments at any of the time points. Albumin reduced vWF concentrations to a larger extent than dextran at both time points whereas no difference in p-vWF GP1bA activity or in plasma fibrinogen and could be detected.</p><p>CONCLUSION: In equipotent doses with regard to plasma volume expansion, dextran-70 transiently prolongs clot amplification time more than albumin whereas dextran-70 reduces plasma vWF concentrations less than albumin.This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0.</p>},
  author       = {Schött, Ulf and Kander, Thomas and Bentzer, Peter},
  issn         = {1540-0514},
  language     = {eng},
  month        = {10},
  pages        = {15},
  publisher    = {BioMedical Press},
  series       = {Shock},
  title        = {Effects of Dextran-70 and Albumin on Coagulation in Experimental Hemorrhage in the Guinea Pig},
  url          = {http://dx.doi.org/10.1097/SHK.0000000000001025},
  year         = {2017},
}