Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis.
Rehn, Gustav LU ; Adlercreutz, Patrick LU
and Grey, Carl
LU
(2014)
In Journal of Biotechnology
179.
p.50-55
- Abstract
- An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of... (More)
- An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-α-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55g/l was reached after 80h, and the system showed promising potential for continuous operation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4379579
- author
- Rehn, Gustav
LU
; Adlercreutz, Patrick
LU
and Grey, Carl
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 179
- pages
- 50 - 55
- publisher
- Elsevier
- external identifiers
-
- pmid:24675224
- wos:000335557800008
- scopus:84897408319
- pmid:24675224
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2014.03.022
- language
- English
- LU publication?
- yes
- id
- 1f83d2ac-d90c-4c64-847b-aaf36794751d (old id 4379579)
- date added to LUP
- 2016-04-01 10:32:15
- date last changed
- 2022-01-26 00:11:19
@article{1f83d2ac-d90c-4c64-847b-aaf36794751d,
abstract = {{An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-α-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55g/l was reached after 80h, and the system showed promising potential for continuous operation.}},
author = {{Rehn, Gustav and Adlercreutz, Patrick and Grey, Carl}},
issn = {{1873-4863}},
language = {{eng}},
pages = {{50--55}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis.}},
url = {{http://dx.doi.org/10.1016/j.jbiotec.2014.03.022}},
doi = {{10.1016/j.jbiotec.2014.03.022}},
volume = {{179}},
year = {{2014}},
}