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Proteomic analysis and discovery using affinity proteomics and mass spectrometry.

Olsson, Niclas LU ; Wingren, Christer LU ; Mattsson, Mikael; James, Peter LU ; O'Connell, David; Nilsson, Fredrik; Cahill, Dolores J and Borrebaeck, Carl LU (2011) In Molecular & Cellular Proteomics 10.
Abstract
Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred... (More)
Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred different proteins. First, the digested proteome is exposed to these antibodies, whereby motif-containing peptides are enriched, which then are detected and identified by mass spectrometry. In this study, we profiled extracts from human colon tissue, yeast cells lysate, and mouse liver tissue to demonstrate proof-of-concept. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular & Cellular Proteomics
volume
10
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000295773800027
  • pmid:21673276
  • scopus:80054021069
ISSN
1535-9484
DOI
10.1074/mcp.M110.003962
project
CREATE Health
language
English
LU publication?
yes
id
eafef29c-9e6a-4c51-a657-0d684dcee9a2 (old id 2008063)
date added to LUP
2011-07-08 09:40:21
date last changed
2017-08-06 03:14:23
@article{eafef29c-9e6a-4c51-a657-0d684dcee9a2,
  abstract     = {Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred different proteins. First, the digested proteome is exposed to these antibodies, whereby motif-containing peptides are enriched, which then are detected and identified by mass spectrometry. In this study, we profiled extracts from human colon tissue, yeast cells lysate, and mouse liver tissue to demonstrate proof-of-concept.},
  author       = {Olsson, Niclas and Wingren, Christer and Mattsson, Mikael and James, Peter and O'Connell, David and Nilsson, Fredrik and Cahill, Dolores J and Borrebaeck, Carl},
  issn         = {1535-9484},
  language     = {eng},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular & Cellular Proteomics},
  title        = {Proteomic analysis and discovery using affinity proteomics and mass spectrometry.},
  url          = {http://dx.doi.org/10.1074/mcp.M110.003962},
  volume       = {10},
  year         = {2011},
}