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The influence of external nitrogen on carbon allocation to Glomus intraradices in monoxenic arbuscular mycorrhiza

Olsson, Pål Axel LU ; Burleigh, Stephen LU and van Aarle, Ingrid LU (2005) In New Phytologist 168(3). p.677-686
Abstract
The influence of external nitrogen (N) on carbon (C) allocation and processes related to phosphorus (P) metabolism were studied in monoxenic arbuscular mycorrhiza (AM) cultures of Daucus carota. Fungal hyphae of Glomus intraradices proliferated from colonized roots growing on solid medium into C-free liquid minimal medium with two different N and P levels. Furthermore, we exposed the colonized roots to high or low N availability and then studied the mycelial development. Roots were provided with C-13-glucose in order to follow the C allocation. The mycelium was analysed for phosphatase activity and transcription levels of two nutrient regulated genes. High N availability to the monoxenic AM root reduced the C allocation to the AM fungus... (More)
The influence of external nitrogen (N) on carbon (C) allocation and processes related to phosphorus (P) metabolism were studied in monoxenic arbuscular mycorrhiza (AM) cultures of Daucus carota. Fungal hyphae of Glomus intraradices proliferated from colonized roots growing on solid medium into C-free liquid minimal medium with two different N and P levels. Furthermore, we exposed the colonized roots to high or low N availability and then studied the mycelial development. Roots were provided with C-13-glucose in order to follow the C allocation. The mycelium was analysed for phosphatase activity and transcription levels of two nutrient regulated genes. High N availability to the monoxenic AM root reduced the C allocation to the AM fungus while N availability to the mycelium was important for the upregulation of the fungal inorganic phosphorus (Pi)-transporter GiPT. We found that N availability can regulate nutritional processes in arbuscular mycorrhiza. We conclude that negative impacts of N on AM abundance are caused by reduced C allocation from the plant. Upregulation of the fungal Pi-transporter GiPT indicated that increased N availability might induce P limitation in the mycelium. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GiPT, metabolic activity, mycorrhiza (AM) cultures, monoxenic atbuscular, phosphorus (P), carbon allocation, nitrogen (N), phosphate transporter
in
New Phytologist
volume
168
issue
3
pages
677 - 686
publisher
Wiley-Blackwell
external identifiers
  • pmid:16313649
  • wos:000233149200018
  • scopus:33645865559
ISSN
1469-8137
DOI
10.1111/j.1469-8137.2005.01532.x
language
English
LU publication?
yes
id
25fcb720-f888-4607-98ce-145327de2806 (old id 213772)
date added to LUP
2007-10-05 11:39:18
date last changed
2017-11-05 03:39:29
@article{25fcb720-f888-4607-98ce-145327de2806,
  abstract     = {The influence of external nitrogen (N) on carbon (C) allocation and processes related to phosphorus (P) metabolism were studied in monoxenic arbuscular mycorrhiza (AM) cultures of Daucus carota. Fungal hyphae of Glomus intraradices proliferated from colonized roots growing on solid medium into C-free liquid minimal medium with two different N and P levels. Furthermore, we exposed the colonized roots to high or low N availability and then studied the mycelial development. Roots were provided with C-13-glucose in order to follow the C allocation. The mycelium was analysed for phosphatase activity and transcription levels of two nutrient regulated genes. High N availability to the monoxenic AM root reduced the C allocation to the AM fungus while N availability to the mycelium was important for the upregulation of the fungal inorganic phosphorus (Pi)-transporter GiPT. We found that N availability can regulate nutritional processes in arbuscular mycorrhiza. We conclude that negative impacts of N on AM abundance are caused by reduced C allocation from the plant. Upregulation of the fungal Pi-transporter GiPT indicated that increased N availability might induce P limitation in the mycelium.},
  author       = {Olsson, Pål Axel and Burleigh, Stephen and van Aarle, Ingrid},
  issn         = {1469-8137},
  keyword      = {GiPT,metabolic activity,mycorrhiza (AM) cultures,monoxenic atbuscular,phosphorus (P),carbon allocation,nitrogen (N),phosphate transporter},
  language     = {eng},
  number       = {3},
  pages        = {677--686},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {The influence of external nitrogen on carbon allocation to Glomus intraradices in monoxenic arbuscular mycorrhiza},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2005.01532.x},
  volume       = {168},
  year         = {2005},
}