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Nuclear localization of {gamma}-tubulin affects E2F transcriptional activity and S-phase progression.

Eklund, Greta LU ; Zarrizi, Reihaneh LU ; von Stedingk, Kristoffer LU ; Jönsson, Kristina and Alvarado-Kristensson, Maria LU (2011) In FASEB Journal 25(11). p.3815-3827
Abstract
We show that the centrosome- and microtubule-regulating protein γ-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. γ-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. γ-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-γ-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of γ-tubulin or by complex formation between γ-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the γ-tubulin C terminus... (More)
We show that the centrosome- and microtubule-regulating protein γ-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. γ-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. γ-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-γ-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of γ-tubulin or by complex formation between γ-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the γ-tubulin C terminus encodes a DNA-binding domain that interacts with E2F-regulated promoters, resulting in γ-tubulin-mediated transient activation of E2Fs. Thus, we report a novel mechanism regulating the activity of E2Fs, which can help explain how these proteins affect cell cycle progression in mammalian cells.-Höög, G., Zarrizi, R., von Stedingk, K., Jonsson, K., Alvarado-Kristensson, M. Nuclear localization of γ-tubulin affects E2F transcriptional activity and S-phase progression. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FASEB Journal
volume
25
issue
11
pages
3815 - 3827
publisher
The Federation of American Societies for Experimental Biology
external identifiers
  • wos:000296723100007
  • pmid:21788450
  • scopus:80355148509
ISSN
1530-6860
DOI
10.1096/fj.11-187484
language
English
LU publication?
yes
id
5a9e4c46-1b94-4181-be2f-eb0cef6ac397 (old id 2151810)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21788450?dopt=Abstract
date added to LUP
2011-09-04 17:15:44
date last changed
2017-08-11 13:45:06
@article{5a9e4c46-1b94-4181-be2f-eb0cef6ac397,
  abstract     = {We show that the centrosome- and microtubule-regulating protein γ-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. γ-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. γ-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-γ-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of γ-tubulin or by complex formation between γ-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the γ-tubulin C terminus encodes a DNA-binding domain that interacts with E2F-regulated promoters, resulting in γ-tubulin-mediated transient activation of E2Fs. Thus, we report a novel mechanism regulating the activity of E2Fs, which can help explain how these proteins affect cell cycle progression in mammalian cells.-Höög, G., Zarrizi, R., von Stedingk, K., Jonsson, K., Alvarado-Kristensson, M. Nuclear localization of γ-tubulin affects E2F transcriptional activity and S-phase progression.},
  author       = {Eklund, Greta and Zarrizi, Reihaneh and von Stedingk, Kristoffer and Jönsson, Kristina and Alvarado-Kristensson, Maria},
  issn         = {1530-6860},
  language     = {eng},
  number       = {11},
  pages        = {3815--3827},
  publisher    = {The Federation of American Societies for Experimental Biology},
  series       = {FASEB Journal},
  title        = {Nuclear localization of {gamma}-tubulin affects E2F transcriptional activity and S-phase progression.},
  url          = {http://dx.doi.org/10.1096/fj.11-187484},
  volume       = {25},
  year         = {2011},
}