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Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media

Kristensson, Elias LU ; Araneo, L.; Berrocal, Edouard LU ; Manin, J.; Richter, Mattias LU ; Aldén, Marcus LU and Linne, M. (2011) In Optics Express 19(14). p.13647-13663
Abstract
The accuracy, precision and limitations of the imaging technique named Structured Laser Illumination Planar Imaging (SLIPI) have been investigated. SLIPI, which allows multiply scattered light to be diminished, has previously demonstrated improvements in image quality and contrast for spray imaging. In the current study the method is applied to a controlled confined environment consisting of a mixture of water and monodisperse polystyrene microspheres. Elastic scattering and fluorescence are studied and the results obtained when probing different particle concentrations and diameters conclusively show the advantages of SLIPI for imaging within moderately turbid media. Although the technique presents both good repeatability and agreement... (More)
The accuracy, precision and limitations of the imaging technique named Structured Laser Illumination Planar Imaging (SLIPI) have been investigated. SLIPI, which allows multiply scattered light to be diminished, has previously demonstrated improvements in image quality and contrast for spray imaging. In the current study the method is applied to a controlled confined environment consisting of a mixture of water and monodisperse polystyrene microspheres. Elastic scattering and fluorescence are studied and the results obtained when probing different particle concentrations and diameters conclusively show the advantages of SLIPI for imaging within moderately turbid media. Although the technique presents both good repeatability and agreement with the Beer-Lambert law, discrepancies in its performance were, however, discovered. Photons undergoing scattering without changing their incident trajectory cannot be discriminated and, owing to differences in scattering phase functions, probing larger particles reduces the suppression of multiply scattered light. However, in terms of visibility such behavior is beneficial as it allows denser media to be probed. It is further demonstrated that the suppression of diffuse light performs equally well regardless of whether photons propagate along the incident direction or towards the camera. In addition, this filtering process acts independently on the spatial distribution of the multiply scattered light but is limited by the finite dynamic range and unavoidable signal noise of the camera. (C) 2011 Optical Society of America (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Optics Express
volume
19
issue
14
pages
13647 - 13663
publisher
OSA
external identifiers
  • wos:000292876500129
  • scopus:79959915646
ISSN
1094-4087
DOI
10.1364/OE.19.013647
language
English
LU publication?
yes
id
580abda0-bb2b-456f-b2f3-51ef0e70b866 (old id 2161899)
date added to LUP
2011-09-21 11:02:37
date last changed
2017-09-10 04:10:22
@article{580abda0-bb2b-456f-b2f3-51ef0e70b866,
  abstract     = {The accuracy, precision and limitations of the imaging technique named Structured Laser Illumination Planar Imaging (SLIPI) have been investigated. SLIPI, which allows multiply scattered light to be diminished, has previously demonstrated improvements in image quality and contrast for spray imaging. In the current study the method is applied to a controlled confined environment consisting of a mixture of water and monodisperse polystyrene microspheres. Elastic scattering and fluorescence are studied and the results obtained when probing different particle concentrations and diameters conclusively show the advantages of SLIPI for imaging within moderately turbid media. Although the technique presents both good repeatability and agreement with the Beer-Lambert law, discrepancies in its performance were, however, discovered. Photons undergoing scattering without changing their incident trajectory cannot be discriminated and, owing to differences in scattering phase functions, probing larger particles reduces the suppression of multiply scattered light. However, in terms of visibility such behavior is beneficial as it allows denser media to be probed. It is further demonstrated that the suppression of diffuse light performs equally well regardless of whether photons propagate along the incident direction or towards the camera. In addition, this filtering process acts independently on the spatial distribution of the multiply scattered light but is limited by the finite dynamic range and unavoidable signal noise of the camera. (C) 2011 Optical Society of America},
  author       = {Kristensson, Elias and Araneo, L. and Berrocal, Edouard and Manin, J. and Richter, Mattias and Aldén, Marcus and Linne, M.},
  issn         = {1094-4087},
  language     = {eng},
  number       = {14},
  pages        = {13647--13663},
  publisher    = {OSA},
  series       = {Optics Express},
  title        = {Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media},
  url          = {http://dx.doi.org/10.1364/OE.19.013647},
  volume       = {19},
  year         = {2011},
}