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Identification of new signaling components in the sensory epithelium of human saccule.

Degerman, Eva LU ; Rauch, Uwe LU ; Göransson, Olga LU ; Lindberg, Sven LU ; Hultgårdh, Anna LU and Magnusson, Måns LU (2011) In Frontiers in Neurology 2.
Abstract
Objective: To locate components and target proteins of relevance for the cAMP and cGMP signaling networks including cAMP and cGMP phosphodiesterases (PDEs), salt-inducible kinases (SIKs), subunits of Na+, K+-ATPases, and aquaporins (AQPs) in the human saccule. Methods: The human saccule was dissected out during the removal of vestibular schwannoma via the translabyrinthine approach and immediately fixed. Immunohistochemistry was performed using PDE, SIK, Na(+), K(+)-ATPase, and AQP antibodies. Results: PDEs selective for cAMP (PDE4A, PDE4D, and PDE8A) and cGMP (PDE9A) as well a dual specificity PDE (PDE10A) were detected in the sensory epithelium of the saccule. Furthermore, AQP2, 4, and 9, SIK1 and the α-1 subunit of the Na(+),... (More)
Objective: To locate components and target proteins of relevance for the cAMP and cGMP signaling networks including cAMP and cGMP phosphodiesterases (PDEs), salt-inducible kinases (SIKs), subunits of Na+, K+-ATPases, and aquaporins (AQPs) in the human saccule. Methods: The human saccule was dissected out during the removal of vestibular schwannoma via the translabyrinthine approach and immediately fixed. Immunohistochemistry was performed using PDE, SIK, Na(+), K(+)-ATPase, and AQP antibodies. Results: PDEs selective for cAMP (PDE4A, PDE4D, and PDE8A) and cGMP (PDE9A) as well a dual specificity PDE (PDE10A) were detected in the sensory epithelium of the saccule. Furthermore, AQP2, 4, and 9, SIK1 and the α-1 subunit of the Na(+), K(+)-ATPase were detected. Conclusion: cAMP and cGMP are important regulators of ion and water homeostasis in the inner ear. The identification of PDEs and SIK1 in the vestibular system offers new treatment targets for endolymphatic hydrops. Exactly how the PDEs are connected to SIK1 and the SIK1 substrate Na(+), K(+)-ATPase and to AQPs 2, 4, 9 remains to be elucidated. The dissection of the signaling networks utilizing these components and evaluating their roles will add new basic knowledge regarding inner ear physiology. (Less)
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organization
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Contribution to journal
publication status
published
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in
Frontiers in Neurology
volume
2
publisher
Frontiers
external identifiers
  • pmid:21886636
  • scopus:84864247532
ISSN
1664-2295
DOI
10.3389/fneur.2011.00048
language
English
LU publication?
yes
id
1ea8188b-bfc8-4489-bd8c-4b197a13294c (old id 2169192)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21886636?dopt=Abstract
date added to LUP
2011-10-03 09:21:15
date last changed
2017-02-12 03:52:33
@article{1ea8188b-bfc8-4489-bd8c-4b197a13294c,
  abstract     = {Objective: To locate components and target proteins of relevance for the cAMP and cGMP signaling networks including cAMP and cGMP phosphodiesterases (PDEs), salt-inducible kinases (SIKs), subunits of Na+, K+-ATPases, and aquaporins (AQPs) in the human saccule. Methods: The human saccule was dissected out during the removal of vestibular schwannoma via the translabyrinthine approach and immediately fixed. Immunohistochemistry was performed using PDE, SIK, Na(+), K(+)-ATPase, and AQP antibodies. Results: PDEs selective for cAMP (PDE4A, PDE4D, and PDE8A) and cGMP (PDE9A) as well a dual specificity PDE (PDE10A) were detected in the sensory epithelium of the saccule. Furthermore, AQP2, 4, and 9, SIK1 and the α-1 subunit of the Na(+), K(+)-ATPase were detected. Conclusion: cAMP and cGMP are important regulators of ion and water homeostasis in the inner ear. The identification of PDEs and SIK1 in the vestibular system offers new treatment targets for endolymphatic hydrops. Exactly how the PDEs are connected to SIK1 and the SIK1 substrate Na(+), K(+)-ATPase and to AQPs 2, 4, 9 remains to be elucidated. The dissection of the signaling networks utilizing these components and evaluating their roles will add new basic knowledge regarding inner ear physiology.},
  articleno    = {48},
  author       = {Degerman, Eva and Rauch, Uwe and Göransson, Olga and Lindberg, Sven and Hultgårdh, Anna and Magnusson, Måns},
  issn         = {1664-2295},
  language     = {eng},
  publisher    = {Frontiers},
  series       = {Frontiers in Neurology},
  title        = {Identification of new signaling components in the sensory epithelium of human saccule.},
  url          = {http://dx.doi.org/10.3389/fneur.2011.00048},
  volume       = {2},
  year         = {2011},
}