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Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis

Larsson, Jonas LU ; Rogstam, Annika LU and von Wachenfeldt, Claes LU (2005) In Microbiology 151(10). p.3323-3335
Abstract
A variety of pathways for electron and carbon flow in the soil bacterium Bacillus subtilis are differentially expressed depending on whether oxygen is present in the cell environment. This study characterizes the regulation of the respiratory oxidase cytochrome bd and the NADH-linked fermentative lactate dehydrogenase, (LDH). Transcription of the cydABCD operon, encoding cytochrome bd, is highly regulated and only becomes activated at low oxygen availability. This induction is not dependent on the gene encoding the redox regulator Fnr or the genes encoding the ResDE two-component regulatory system. The DNA-binding protein YdiH was found to be a principal regulator that controls cydABCD expression. Transcription from the cyd promoter is... (More)
A variety of pathways for electron and carbon flow in the soil bacterium Bacillus subtilis are differentially expressed depending on whether oxygen is present in the cell environment. This study characterizes the regulation of the respiratory oxidase cytochrome bd and the NADH-linked fermentative lactate dehydrogenase, (LDH). Transcription of the cydABCD operon, encoding cytochrome bd, is highly regulated and only becomes activated at low oxygen availability. This induction is not dependent on the gene encoding the redox regulator Fnr or the genes encoding the ResDE two-component regulatory system. The DNA-binding protein YdiH was found to be a principal regulator that controls cydABCD expression. Transcription from the cyd promoter is stimulated 15-fold by a region located upstream of the core promoter. The upstream region may constitute a binding site for an unidentified transcription activator that is likely to influence the level of transcription but not its timing, which is negatively controlled by YdiH. This report provides evidence that YdiH also functions as a repressor of the ldh gene encoding LDH and of a gene, ywcJ, which encodes a putative formate-nitrite transporter. Based on the similarity between YdiH and the Rex protein of Streptomyces coelicolor, it is proposed that YdiH serves as a redox sensor, the activity of which is regulated by cellular differences in the free levels of NAD(+) and NADH. It is suggested that ydiH be renamed as rex. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbiology
volume
151
issue
10
pages
3323 - 3335
publisher
MAIK Nauka/Interperiodica
external identifiers
  • pmid:16207915
  • wos:000232551300018
  • scopus:27144540194
ISSN
1465-2080
DOI
10.1099/mic.0.28124-0
language
English
LU publication?
yes
id
32709f76-46e3-4ebe-aca8-2ec221ad4c26 (old id 220119)
date added to LUP
2007-08-07 15:30:11
date last changed
2017-10-22 03:52:07
@article{32709f76-46e3-4ebe-aca8-2ec221ad4c26,
  abstract     = {A variety of pathways for electron and carbon flow in the soil bacterium Bacillus subtilis are differentially expressed depending on whether oxygen is present in the cell environment. This study characterizes the regulation of the respiratory oxidase cytochrome bd and the NADH-linked fermentative lactate dehydrogenase, (LDH). Transcription of the cydABCD operon, encoding cytochrome bd, is highly regulated and only becomes activated at low oxygen availability. This induction is not dependent on the gene encoding the redox regulator Fnr or the genes encoding the ResDE two-component regulatory system. The DNA-binding protein YdiH was found to be a principal regulator that controls cydABCD expression. Transcription from the cyd promoter is stimulated 15-fold by a region located upstream of the core promoter. The upstream region may constitute a binding site for an unidentified transcription activator that is likely to influence the level of transcription but not its timing, which is negatively controlled by YdiH. This report provides evidence that YdiH also functions as a repressor of the ldh gene encoding LDH and of a gene, ywcJ, which encodes a putative formate-nitrite transporter. Based on the similarity between YdiH and the Rex protein of Streptomyces coelicolor, it is proposed that YdiH serves as a redox sensor, the activity of which is regulated by cellular differences in the free levels of NAD(+) and NADH. It is suggested that ydiH be renamed as rex.},
  author       = {Larsson, Jonas and Rogstam, Annika and von Wachenfeldt, Claes},
  issn         = {1465-2080},
  language     = {eng},
  number       = {10},
  pages        = {3323--3335},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology},
  title        = {Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis},
  url          = {http://dx.doi.org/10.1099/mic.0.28124-0},
  volume       = {151},
  year         = {2005},
}