Advanced

In vitro Mutagen binding and antimutagenic activity of human Lactobacillus rhamnosus 231

Ambalam, Padma; Dave, J. M.; Nair, Baboo M LU and Vyas, B. R. M. (2011) In Anaerobe 17(5). p.217-222
Abstract
In vitro mutagen binding ability of human Lactobacillus rhamnosus 231 (Lr 231) was evaluated against acridine orange (AO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 2-amino-3, 8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx) and 4-nitro-o-phenylenediamine (NPD). Binding of AO by Lr 231 is due to adsorption, thereby leading to removal of mutagen in solution and is instantaneous, pH- and concentration-dependent. Whereas, binding of MNNG and MeIQx by Lr 231 results into biotransformation leading to detoxification with subsequent loss of mutagenicity as determined by spectral analysis, thin layer chromatography and Ames test. Binding of mutagen by Lr 231 was dependent on culture age and optimum binding of AO, MNNG and MeIQx was observed to... (More)
In vitro mutagen binding ability of human Lactobacillus rhamnosus 231 (Lr 231) was evaluated against acridine orange (AO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 2-amino-3, 8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx) and 4-nitro-o-phenylenediamine (NPD). Binding of AO by Lr 231 is due to adsorption, thereby leading to removal of mutagen in solution and is instantaneous, pH- and concentration-dependent. Whereas, binding of MNNG and MeIQx by Lr 231 results into biotransformation leading to detoxification with subsequent loss of mutagenicity as determined by spectral analysis, thin layer chromatography and Ames test. Binding of mutagen by Lr 231 was dependent on culture age and optimum binding of AO, MNNG and MeIQx was observed to occur with 24 h old culture. Cells of Lr 231 were subjected to different chemical treatments prior to binding studies. Results indicated cell wall component such as cell wall polysaccharide, peptidoglycan, carbohydrates and proteins plays an important role in adsorption of AO, also involving hydrophilic and ionic interactions. Binding, biotransformation and detoxification of MNNG and MeIQx by Lr 231 was dependent on cell surface characteristics mainly involving carbohydrates, proteins, teichoic acid/lipoteichoic acid, hydrophobic interaction and presence of thiol group. L rhamnosus 231 bound MNNG instantaneously. More than 96 (p < 0.01) and 70% (p < 0.05) cells remained viable after mutagen binding and various pretreatments respectively. This study shows Lr 231 exhibits ability to bind and detoxify potent mutagens, and this property can be useful in formulating fermented foods for removal of potent mutagens. (C) 2011 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acridine orange, Antimutagens, Biotransformation, Detoxification, Lactobacillus rhamnosus, MNNG, MeIQX
in
Anaerobe
volume
17
issue
5
pages
217 - 222
publisher
Elsevier
external identifiers
  • wos:000296118100001
  • scopus:80053121145
ISSN
1095-8274
DOI
10.1016/j.anaerobe.2011.07.001
language
English
LU publication?
yes
id
aad3fb96-6f4d-4f09-90d5-f20aa3d0d007 (old id 2211618)
date added to LUP
2011-11-30 11:42:11
date last changed
2017-05-14 03:09:29
@article{aad3fb96-6f4d-4f09-90d5-f20aa3d0d007,
  abstract     = {In vitro mutagen binding ability of human Lactobacillus rhamnosus 231 (Lr 231) was evaluated against acridine orange (AO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 2-amino-3, 8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx) and 4-nitro-o-phenylenediamine (NPD). Binding of AO by Lr 231 is due to adsorption, thereby leading to removal of mutagen in solution and is instantaneous, pH- and concentration-dependent. Whereas, binding of MNNG and MeIQx by Lr 231 results into biotransformation leading to detoxification with subsequent loss of mutagenicity as determined by spectral analysis, thin layer chromatography and Ames test. Binding of mutagen by Lr 231 was dependent on culture age and optimum binding of AO, MNNG and MeIQx was observed to occur with 24 h old culture. Cells of Lr 231 were subjected to different chemical treatments prior to binding studies. Results indicated cell wall component such as cell wall polysaccharide, peptidoglycan, carbohydrates and proteins plays an important role in adsorption of AO, also involving hydrophilic and ionic interactions. Binding, biotransformation and detoxification of MNNG and MeIQx by Lr 231 was dependent on cell surface characteristics mainly involving carbohydrates, proteins, teichoic acid/lipoteichoic acid, hydrophobic interaction and presence of thiol group. L rhamnosus 231 bound MNNG instantaneously. More than 96 (p &lt; 0.01) and 70% (p &lt; 0.05) cells remained viable after mutagen binding and various pretreatments respectively. This study shows Lr 231 exhibits ability to bind and detoxify potent mutagens, and this property can be useful in formulating fermented foods for removal of potent mutagens. (C) 2011 Elsevier Ltd. All rights reserved.},
  author       = {Ambalam, Padma and Dave, J. M. and Nair, Baboo M and Vyas, B. R. M.},
  issn         = {1095-8274},
  keyword      = {Acridine orange,Antimutagens,Biotransformation,Detoxification,Lactobacillus rhamnosus,MNNG,MeIQX},
  language     = {eng},
  number       = {5},
  pages        = {217--222},
  publisher    = {Elsevier},
  series       = {Anaerobe},
  title        = {In vitro Mutagen binding and antimutagenic activity of human Lactobacillus rhamnosus 231},
  url          = {http://dx.doi.org/10.1016/j.anaerobe.2011.07.001},
  volume       = {17},
  year         = {2011},
}