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Effectiveness of ciprofloxacin or linezolid in combination with rifampicin against Enterococcus faecalis in biofilms.

Holmberg, Anna LU ; Mörgelin, Matthias LU and Rasmussen, Magnus LU (2012) In Journal of Antimicrobial Chemotherapy 67. p.433-439
Abstract
ObjectivesTo assess the in vitro antibiotic susceptibility of Enterococcus faecalis in biofilms.MethodsE. faecalis isolates were from infections of prosthetic hip and knee joints. Minimal biofilm eradication concentrations (MBECs) for ampicillin, vancomycin, linezolid, ciprofloxacin and rifampicin, alone and in combinations, were determined. E. faecalis biofilms in microtitre plate wells or on beads of bone cement were treated with the same antibiotics for various times followed by plating to quantify surviving bacteria.ResultsThe E. faecalis isolates displayed MBECs for the various antibiotics of between 64 and 512 mg/L. When combined with 8 mg/L rifampicin, MBECs for ciprofloxacin and linezolid dropped to 16-32 mg/L for the four isolates... (More)
ObjectivesTo assess the in vitro antibiotic susceptibility of Enterococcus faecalis in biofilms.MethodsE. faecalis isolates were from infections of prosthetic hip and knee joints. Minimal biofilm eradication concentrations (MBECs) for ampicillin, vancomycin, linezolid, ciprofloxacin and rifampicin, alone and in combinations, were determined. E. faecalis biofilms in microtitre plate wells or on beads of bone cement were treated with the same antibiotics for various times followed by plating to quantify surviving bacteria.ResultsThe E. faecalis isolates displayed MBECs for the various antibiotics of between 64 and 512 mg/L. When combined with 8 mg/L rifampicin, MBECs for ciprofloxacin and linezolid dropped to 16-32 mg/L for the four isolates tested. In biofilms formed on a plastic surface and on bone cement beads significant bacterial killing (>2-3 log decrease in cfus) was seen after an 8 h exposure to antibiotics. For all isolates, the combination of ciprofloxacin and rifampicin, followed by linezolid and rifampicin, was most effective in reducing the number of bacteria. E. faecalis developed resistance to rifampicin in our system when biofilms were subjected to this antibiotic, alone or in combination with ampicillin. When bacteria were subjected to combinations with ciprofloxacin or linezolid development of resistance was reduced.ConclusionsOur results show that combinations of ciprofloxacin or linezolid with rifampicin have a good effect on E. faecalis biofilms in vitro. These combinations should be considered for testing in humans with early joint prosthesis infections with E. faecalis. (Less)
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Contribution to journal
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published
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in
Journal of Antimicrobial Chemotherapy
volume
67
pages
433 - 439
publisher
Oxford University Press
external identifiers
  • wos:000299096600022
  • pmid:22110086
  • scopus:84855822708
ISSN
1460-2091
DOI
10.1093/jac/dkr477
language
English
LU publication?
yes
id
f3165de3-d040-45c3-9c09-12167ee4a35e (old id 2220487)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22110086?dopt=Abstract
date added to LUP
2011-12-03 09:36:17
date last changed
2017-10-22 04:56:17
@article{f3165de3-d040-45c3-9c09-12167ee4a35e,
  abstract     = {ObjectivesTo assess the in vitro antibiotic susceptibility of Enterococcus faecalis in biofilms.MethodsE. faecalis isolates were from infections of prosthetic hip and knee joints. Minimal biofilm eradication concentrations (MBECs) for ampicillin, vancomycin, linezolid, ciprofloxacin and rifampicin, alone and in combinations, were determined. E. faecalis biofilms in microtitre plate wells or on beads of bone cement were treated with the same antibiotics for various times followed by plating to quantify surviving bacteria.ResultsThe E. faecalis isolates displayed MBECs for the various antibiotics of between 64 and 512 mg/L. When combined with 8 mg/L rifampicin, MBECs for ciprofloxacin and linezolid dropped to 16-32 mg/L for the four isolates tested. In biofilms formed on a plastic surface and on bone cement beads significant bacterial killing (>2-3 log decrease in cfus) was seen after an 8 h exposure to antibiotics. For all isolates, the combination of ciprofloxacin and rifampicin, followed by linezolid and rifampicin, was most effective in reducing the number of bacteria. E. faecalis developed resistance to rifampicin in our system when biofilms were subjected to this antibiotic, alone or in combination with ampicillin. When bacteria were subjected to combinations with ciprofloxacin or linezolid development of resistance was reduced.ConclusionsOur results show that combinations of ciprofloxacin or linezolid with rifampicin have a good effect on E. faecalis biofilms in vitro. These combinations should be considered for testing in humans with early joint prosthesis infections with E. faecalis.},
  author       = {Holmberg, Anna and Mörgelin, Matthias and Rasmussen, Magnus},
  issn         = {1460-2091},
  language     = {eng},
  pages        = {433--439},
  publisher    = {Oxford University Press},
  series       = {Journal of Antimicrobial Chemotherapy},
  title        = {Effectiveness of ciprofloxacin or linezolid in combination with rifampicin against Enterococcus faecalis in biofilms.},
  url          = {http://dx.doi.org/10.1093/jac/dkr477},
  volume       = {67},
  year         = {2012},
}