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Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation

Ramos, Yassel ; González, Annia ; Sosa-Acosta, Patricia ; Perez-Riverol, Yasset ; García, Yairet ; Castellanos-Serra, Lila ; Gil, Jeovanis LU ; Sánchez, Aniel LU ; González, Luis J. and Besada, Vladimir (2019) In Journal of Separation Science
Abstract

Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free... (More)

Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
electrophoresis, peptide fractionation, proteomics
in
Journal of Separation Science
publisher
John Wiley & Sons
external identifiers
  • pmid:31625274
  • scopus:85074854756
ISSN
1615-9306
DOI
10.1002/jssc.201900495
language
English
LU publication?
yes
id
2236566e-7425-4ae6-85f5-472ecf32da12
date added to LUP
2019-12-02 14:39:14
date last changed
2019-12-03 02:21:17
@article{2236566e-7425-4ae6-85f5-472ecf32da12,
  abstract     = {<p>Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.</p>},
  author       = {Ramos, Yassel and González, Annia and Sosa-Acosta, Patricia and Perez-Riverol, Yasset and García, Yairet and Castellanos-Serra, Lila and Gil, Jeovanis and Sánchez, Aniel and González, Luis J. and Besada, Vladimir},
  issn         = {1615-9306},
  language     = {eng},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation},
  url          = {http://dx.doi.org/10.1002/jssc.201900495},
  doi          = {10.1002/jssc.201900495},
  year         = {2019},
}