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Influence of mast cells on the expression of adhesion molecules on circulating and migrating leukocytes in acute pancreatitis-associated lung injury

Zhao, Xia LU ; Dib, Marwan LU ; Wang, Xiangdong LU ; Widegren, Bengt LU and Andersson, Roland LU (2005) In Lung 183(4). p.253-264
Abstract
Pancreatitis-associated lung injury is an early-occurring and severe complication, still associated with substantial mortality. A number of inflammatory cells and their products are involved in the initiation and progress of the condition. In the present study, acute pancreatitis (AP) was induced by the intraductal infusion of 5% sodium taurodeoxycholate in the rat. Pulmonary endothelial barrier dysfunction was measured by plasma exudation of radiolabeled albumin. Expression of PECAM-1, ICAM-1, and L-selectin on neutrophils (CD11b(+)) and monocytes/macrophages (CD11b/c(+)), obtained from circulation and lung tissue, was measured I and 6 hours after AP induction (n = 10 rats/time point/group). Plasma levels of histamine and serotonin were... (More)
Pancreatitis-associated lung injury is an early-occurring and severe complication, still associated with substantial mortality. A number of inflammatory cells and their products are involved in the initiation and progress of the condition. In the present study, acute pancreatitis (AP) was induced by the intraductal infusion of 5% sodium taurodeoxycholate in the rat. Pulmonary endothelial barrier dysfunction was measured by plasma exudation of radiolabeled albumin. Expression of PECAM-1, ICAM-1, and L-selectin on neutrophils (CD11b(+)) and monocytes/macrophages (CD11b/c(+)), obtained from circulation and lung tissue, was measured I and 6 hours after AP induction (n = 10 rats/time point/group). Plasma levels of histamine and serotonin were determined. The role of mast cells was evaluated by pretreatment with the mast cell stabilizer cromolyn. Intraperitoneal administration of cromolyn downregulated pancreatitis-induced systemic increase of histamine at I hour (513 82 vs. 309 50, p < 0.05). Cromolyn prevented a decreased expression of PECAM-I on circulatory neutrophils and monocytes/macrophages and against an increased expression of ICAM-1 and PECAM-1 on pulmonary neutrophils and monocytes/ macrophages 6 hours after AP induction (about 40% vs. 10%, p < 0.01). The mast cell stabilizer also prevented pancreatitis-induced pulmonary endothelial barrier dysfunction at 6 hours. Thus, our data indicate that mast cells may play a critical role in the activation of leukocytes during the initiation of pancreatitis-associated lung injury by altering phenotypes of adhesion molecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
histamine adhesion molecules, mast cells, cromolyn, permeability
in
Lung
volume
183
issue
4
pages
253 - 264
publisher
Springer
external identifiers
  • wos:000231468700003
  • scopus:26444586689
ISSN
1432-1750
DOI
10.1007/s00408-004-2538-8
language
English
LU publication?
yes
id
f1cf9fb1-7466-4d75-9fca-947beb97bb07 (old id 226673)
date added to LUP
2007-08-15 16:39:46
date last changed
2017-05-28 04:17:50
@article{f1cf9fb1-7466-4d75-9fca-947beb97bb07,
  abstract     = {Pancreatitis-associated lung injury is an early-occurring and severe complication, still associated with substantial mortality. A number of inflammatory cells and their products are involved in the initiation and progress of the condition. In the present study, acute pancreatitis (AP) was induced by the intraductal infusion of 5% sodium taurodeoxycholate in the rat. Pulmonary endothelial barrier dysfunction was measured by plasma exudation of radiolabeled albumin. Expression of PECAM-1, ICAM-1, and L-selectin on neutrophils (CD11b(+)) and monocytes/macrophages (CD11b/c(+)), obtained from circulation and lung tissue, was measured I and 6 hours after AP induction (n = 10 rats/time point/group). Plasma levels of histamine and serotonin were determined. The role of mast cells was evaluated by pretreatment with the mast cell stabilizer cromolyn. Intraperitoneal administration of cromolyn downregulated pancreatitis-induced systemic increase of histamine at I hour (513 82 vs. 309 50, p &lt; 0.05). Cromolyn prevented a decreased expression of PECAM-I on circulatory neutrophils and monocytes/macrophages and against an increased expression of ICAM-1 and PECAM-1 on pulmonary neutrophils and monocytes/ macrophages 6 hours after AP induction (about 40% vs. 10%, p &lt; 0.01). The mast cell stabilizer also prevented pancreatitis-induced pulmonary endothelial barrier dysfunction at 6 hours. Thus, our data indicate that mast cells may play a critical role in the activation of leukocytes during the initiation of pancreatitis-associated lung injury by altering phenotypes of adhesion molecules.},
  author       = {Zhao, Xia and Dib, Marwan and Wang, Xiangdong and Widegren, Bengt and Andersson, Roland},
  issn         = {1432-1750},
  keyword      = {histamine adhesion molecules,mast cells,cromolyn,permeability},
  language     = {eng},
  number       = {4},
  pages        = {253--264},
  publisher    = {Springer},
  series       = {Lung},
  title        = {Influence of mast cells on the expression of adhesion molecules on circulating and migrating leukocytes in acute pancreatitis-associated lung injury},
  url          = {http://dx.doi.org/10.1007/s00408-004-2538-8},
  volume       = {183},
  year         = {2005},
}