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Asymmetrical flow field-flow fractionation enables the characterization of molecular and supramolecular properties of cereal beta-glucan dispersions

Håkansson, Andreas LU ; Ulmius, Matilda LU and Nilsson, Lars LU (2012) In Carbohydrate Polymers 87(1). p.518-523
Abstract
In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over... (More)
In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over the entire size distribution. Small species in the barley beta-glucan samples display properties ranging from elongated conformation to random coil conformation. Aggregates have

low apparent densities and a swollen micro gel structure. Oat beta-glucan displays no properties that can be attributed to a molecularly dissolved beta-glucan showing that dissolution was incomplete. The aggregate properties analyzed were similar between oat and barley beta-glucan. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Beta-glucan Asymmetrical flow field-flow fractionation AF4 Oat Barley
in
Carbohydrate Polymers
volume
87
issue
1
pages
518 - 523
publisher
Elsevier
external identifiers
  • wos:000297389000068
  • scopus:80054737009
ISSN
0144-8617
DOI
10.1016/j.carbpol.2011.08.014
language
English
LU publication?
yes
id
8cbfbea0-196f-4f71-8069-16afcbc260ac (old id 2277730)
date added to LUP
2012-01-09 09:14:19
date last changed
2017-09-24 03:07:23
@article{8cbfbea0-196f-4f71-8069-16afcbc260ac,
  abstract     = {In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over the entire size distribution. Small species in the barley beta-glucan samples display properties ranging from elongated conformation to random coil conformation. Aggregates have<br/><br>
low apparent densities and a swollen micro gel structure. Oat beta-glucan displays no properties that can be attributed to a molecularly dissolved beta-glucan showing that dissolution was incomplete. The aggregate properties analyzed were similar between oat and barley beta-glucan.},
  author       = {Håkansson, Andreas and Ulmius, Matilda and Nilsson, Lars},
  issn         = {0144-8617},
  keyword      = {Beta-glucan Asymmetrical flow field-flow fractionation AF4 Oat Barley},
  language     = {eng},
  number       = {1},
  pages        = {518--523},
  publisher    = {Elsevier},
  series       = {Carbohydrate Polymers},
  title        = {Asymmetrical flow field-flow fractionation enables the characterization of molecular and supramolecular properties of cereal beta-glucan dispersions},
  url          = {http://dx.doi.org/10.1016/j.carbpol.2011.08.014},
  volume       = {87},
  year         = {2012},
}