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The calpain inhibitor VI prevents apoptosis of adult motor neurons

Momeni, HamidReza LU and Kanje, Martin LU (2005) In NeuroReport 16(10). p.1065-1068
Abstract
Motor neuron cell death was studied in organotypic cultures of adult spinal cord slices from the mouse. Six hours after excision, many motor neuron nuclei displayed apoptotic features including nuclear and chromatin condensation. At this time point, many motor neurons also exhibited immunoreactivity to calpain II. Both the calpain inhibitor VI and ethyleneglycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) prevented the appearance of apoptotic nuclei whereas the pan caspase inhibitor Z-VAD.fmk had no effect. The results suggest that calpain is required for apoptosis of motor neurons and that this type of apoptosis is independent of caspase activation.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
culture, organ, motor neurons, caspase, calpain inhibitor, apoptosis, calpain, spinal cord
in
NeuroReport
volume
16
issue
10
pages
1065 - 1068
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:15973149
  • wos:000230772100007
  • scopus:22544456011
ISSN
1473-558X
language
English
LU publication?
yes
id
4367428f-213b-4e7b-b21c-7726b5a94cd9 (old id 231983)
date added to LUP
2007-10-05 09:52:27
date last changed
2017-01-01 05:06:02
@article{4367428f-213b-4e7b-b21c-7726b5a94cd9,
  abstract     = {Motor neuron cell death was studied in organotypic cultures of adult spinal cord slices from the mouse. Six hours after excision, many motor neuron nuclei displayed apoptotic features including nuclear and chromatin condensation. At this time point, many motor neurons also exhibited immunoreactivity to calpain II. Both the calpain inhibitor VI and ethyleneglycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) prevented the appearance of apoptotic nuclei whereas the pan caspase inhibitor Z-VAD.fmk had no effect. The results suggest that calpain is required for apoptosis of motor neurons and that this type of apoptosis is independent of caspase activation.},
  author       = {Momeni, HamidReza and Kanje, Martin},
  issn         = {1473-558X},
  keyword      = {culture,organ,motor neurons,caspase,calpain inhibitor,apoptosis,calpain,spinal cord},
  language     = {eng},
  number       = {10},
  pages        = {1065--1068},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {The calpain inhibitor VI prevents apoptosis of adult motor neurons},
  volume       = {16},
  year         = {2005},
}