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Effect of murine strain on metabolic pathways of glucose production after brief or prolonged fasting

Burgess, SC; Jeffrey, FMH; Storey, C; Milde, A; Hausler, N; Merritt, ME; Mulder, Hindrik LU ; Holm, Cecilia LU ; Sherry, AD and Malloy, CR (2005) In American Journal of Physiology: Endocrinology and Metabolism 289(1). p.53-61
Abstract
Background strain is known to influence the way a genetic manipulation affects mouse phenotypes. Despite data that demonstrate variations in the primary phenotype of basic inbred strains of mice, there is limited data available about specific metabolic fluxes in vivo that may be responsible for the differences in strain phenotypes. In this study, a simple stable isotope tracer/NMR spectroscopic protocol has been used to compare metabolic fluxes in ICR, FVB/N (FVB), C57BL/6J (B6), and 129S1/SvImJ (129) mouse strains. After a short-term fast in these mice, there were no detectable differences in the pathway fluxes that contribute to glucose synthesis. However, after a 24-h fast, B6 mice retain some residual glycogenolysis compared with other... (More)
Background strain is known to influence the way a genetic manipulation affects mouse phenotypes. Despite data that demonstrate variations in the primary phenotype of basic inbred strains of mice, there is limited data available about specific metabolic fluxes in vivo that may be responsible for the differences in strain phenotypes. In this study, a simple stable isotope tracer/NMR spectroscopic protocol has been used to compare metabolic fluxes in ICR, FVB/N (FVB), C57BL/6J (B6), and 129S1/SvImJ (129) mouse strains. After a short-term fast in these mice, there were no detectable differences in the pathway fluxes that contribute to glucose synthesis. However, after a 24-h fast, B6 mice retain some residual glycogenolysis compared with other strains. FVB mice also had a 30% higher in vivo phosphoenolpyruvate carboxykinase flux and total glucose production from the level of the TCA cycle compared with B6 and 129 strains, while total body glucose production in the 129 strain was similar to 30% lower than in either FVB or B6 mice. These data indicate that there are inherent differences in several pathways involving glucose metabolism of inbred strains of mice that may contribute to a phenotype after genetic manipulation in these animals. The techniques used here are amenable to use as a secondary or tertiary tool for studying mouse models with disruptions of intermediary metabolism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
stable isotope, metabolic flux, phosphoenolpyruvate carboxykinase, nuclear magnetic resonance, tricarboxylic acid cycle, tracers, deuterium, mouse phenotype
in
American Journal of Physiology: Endocrinology and Metabolism
volume
289
issue
1
pages
53 - 61
publisher
American Physiological Society
external identifiers
  • wos:000230113600009
  • pmid:15797985
  • scopus:21044438041
ISSN
1522-1555
DOI
10.1152/ajpendo.00601.2004
language
English
LU publication?
yes
id
69c3b89f-7817-4f3d-84de-2b8870863a6a (old id 234022)
date added to LUP
2007-08-10 11:10:39
date last changed
2017-11-05 04:30:33
@article{69c3b89f-7817-4f3d-84de-2b8870863a6a,
  abstract     = {Background strain is known to influence the way a genetic manipulation affects mouse phenotypes. Despite data that demonstrate variations in the primary phenotype of basic inbred strains of mice, there is limited data available about specific metabolic fluxes in vivo that may be responsible for the differences in strain phenotypes. In this study, a simple stable isotope tracer/NMR spectroscopic protocol has been used to compare metabolic fluxes in ICR, FVB/N (FVB), C57BL/6J (B6), and 129S1/SvImJ (129) mouse strains. After a short-term fast in these mice, there were no detectable differences in the pathway fluxes that contribute to glucose synthesis. However, after a 24-h fast, B6 mice retain some residual glycogenolysis compared with other strains. FVB mice also had a 30% higher in vivo phosphoenolpyruvate carboxykinase flux and total glucose production from the level of the TCA cycle compared with B6 and 129 strains, while total body glucose production in the 129 strain was similar to 30% lower than in either FVB or B6 mice. These data indicate that there are inherent differences in several pathways involving glucose metabolism of inbred strains of mice that may contribute to a phenotype after genetic manipulation in these animals. The techniques used here are amenable to use as a secondary or tertiary tool for studying mouse models with disruptions of intermediary metabolism.},
  author       = {Burgess, SC and Jeffrey, FMH and Storey, C and Milde, A and Hausler, N and Merritt, ME and Mulder, Hindrik and Holm, Cecilia and Sherry, AD and Malloy, CR},
  issn         = {1522-1555},
  keyword      = {stable isotope,metabolic flux,phosphoenolpyruvate carboxykinase,nuclear magnetic resonance,tricarboxylic acid cycle,tracers,deuterium,mouse phenotype},
  language     = {eng},
  number       = {1},
  pages        = {53--61},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology: Endocrinology and Metabolism},
  title        = {Effect of murine strain on metabolic pathways of glucose production after brief or prolonged fasting},
  url          = {http://dx.doi.org/10.1152/ajpendo.00601.2004},
  volume       = {289},
  year         = {2005},
}