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Development of silicon microstructures and thin-film MALDI target plates for automated proteomics sample identifications

Miliotis, T.; Marko-Varga, György LU ; Nilsson, J. and Laurell, Thomas LU (2001) In Journal of Neuroscience Methods 109(1). p.41-46
Abstract
Here we report on the development of a proteomic platform utilizing a piezoelectric flow-through dispensing unit made from silicon microstructures. The use of a novel surface coating, where matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI MS) targets were uniformly precoated with a thin film of matrix/nitrocellulose, made the sample preparation straightforward and enabled the enrichment and analysis of proteins at low levels in proteomics samples. We demonstrate this by analyzing excised spots in a biological sample originating from a human fetal fibroblast cell line that was subjected to 2D gel-electrophoresis. Furthermore, a sample deposition rate below 30 Hz results in an increased analyte density on... (More)
Here we report on the development of a proteomic platform utilizing a piezoelectric flow-through dispensing unit made from silicon microstructures. The use of a novel surface coating, where matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI MS) targets were uniformly precoated with a thin film of matrix/nitrocellulose, made the sample preparation straightforward and enabled the enrichment and analysis of proteins at low levels in proteomics samples. We demonstrate this by analyzing excised spots in a biological sample originating from a human fetal fibroblast cell line that was subjected to 2D gel-electrophoresis. Furthermore, a sample deposition rate below 30 Hz results in an increased analyte density on the dispensed sample spot, rendering signal amplification. In general, the sensitivity for proteins and peptides can be enhanced 10-50 times compared to traditional MALDI sample preparation techniques. (C) 2001 Elsevier Science B.V. All rights reserved. (Less)
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Contribution to journal
publication status
published
subject
keywords
flow-through microdispenser, precoated target plate, MALDI-TOF MS, proteins, ASSISTED-LASER-DESORPTION/IONIZATION, FLIGHT MASS-SPECTROMETRY, PROTEIN, IDENTIFICATION, FUNCTIONAL PROTEOMICS, SIGNAL-TRANSDUCTION, REPRODUCIBILITY, GELS
in
Journal of Neuroscience Methods
volume
109
issue
1
pages
41 - 46
publisher
Elsevier
external identifiers
  • wos:000171197700006
  • scopus:0035882298
ISSN
1872-678X
DOI
10.1016/s0165-0270(01)00399-5
language
English
LU publication?
yes
id
d048dceb-1194-4757-9586-8ae67be68388 (old id 2376410)
date added to LUP
2012-03-23 09:13:09
date last changed
2018-05-29 11:34:34
@article{d048dceb-1194-4757-9586-8ae67be68388,
  abstract     = {Here we report on the development of a proteomic platform utilizing a piezoelectric flow-through dispensing unit made from silicon microstructures. The use of a novel surface coating, where matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI MS) targets were uniformly precoated with a thin film of matrix/nitrocellulose, made the sample preparation straightforward and enabled the enrichment and analysis of proteins at low levels in proteomics samples. We demonstrate this by analyzing excised spots in a biological sample originating from a human fetal fibroblast cell line that was subjected to 2D gel-electrophoresis. Furthermore, a sample deposition rate below 30 Hz results in an increased analyte density on the dispensed sample spot, rendering signal amplification. In general, the sensitivity for proteins and peptides can be enhanced 10-50 times compared to traditional MALDI sample preparation techniques. (C) 2001 Elsevier Science B.V. All rights reserved.},
  author       = {Miliotis, T. and Marko-Varga, György and Nilsson, J. and Laurell, Thomas},
  issn         = {1872-678X},
  keyword      = {flow-through microdispenser,precoated target plate,MALDI-TOF MS,proteins,ASSISTED-LASER-DESORPTION/IONIZATION,FLIGHT MASS-SPECTROMETRY,PROTEIN,IDENTIFICATION,FUNCTIONAL PROTEOMICS,SIGNAL-TRANSDUCTION,REPRODUCIBILITY,GELS},
  language     = {eng},
  number       = {1},
  pages        = {41--46},
  publisher    = {Elsevier},
  series       = {Journal of Neuroscience Methods},
  title        = {Development of silicon microstructures and thin-film MALDI target plates for automated proteomics sample identifications},
  url          = {http://dx.doi.org/10.1016/s0165-0270(01)00399-5},
  volume       = {109},
  year         = {2001},
}