First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study
(2019) In Analytical Chemistry 91(11). p.6953-6961- Abstract
- The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of... (More)
- The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results. © 2019 American Chemical Society. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/23c3c90f-54f2-4c24-9fd5-2003aee760be
- author
- Iacobucci, C. ; Bernfur, Katja LU ; Emanuelsson, Cecilia LU ; Leitner, A. and Sinz, A.
- author collaboration
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Crosslinking, Data handling, Information analysis, Mass spectrometry, Molecular biology, Best practice guidelines, Chemical cross-linking, Community-based, Protein-protein interactions, Reporting formats, Status quo, Structural biology, Three dimensional structure modeling, Proteins
- in
- Analytical Chemistry
- volume
- 91
- issue
- 11
- pages
- 9 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85066804197
- pmid:31045356
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.9b00658
- language
- English
- LU publication?
- yes
- id
- 23c3c90f-54f2-4c24-9fd5-2003aee760be
- date added to LUP
- 2019-07-24 08:58:45
- date last changed
- 2022-04-26 03:15:35
@article{23c3c90f-54f2-4c24-9fd5-2003aee760be, abstract = {{The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results. © 2019 American Chemical Society.}}, author = {{Iacobucci, C. and Bernfur, Katja and Emanuelsson, Cecilia and Leitner, A. and Sinz, A.}}, issn = {{1520-6882}}, keywords = {{Crosslinking; Data handling; Information analysis; Mass spectrometry; Molecular biology; Best practice guidelines; Chemical cross-linking; Community-based; Protein-protein interactions; Reporting formats; Status quo; Structural biology; Three dimensional structure modeling; Proteins}}, language = {{eng}}, number = {{11}}, pages = {{6953--6961}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study}}, url = {{http://dx.doi.org/10.1021/acs.analchem.9b00658}}, doi = {{10.1021/acs.analchem.9b00658}}, volume = {{91}}, year = {{2019}}, }