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Loss of Regulator of G-Protein Signaling 5 Leads to Neurovascular Protection in Stroke

Özen, Ilknur LU ; Roth, Michaela LU ; Barbariga, Marco LU ; Gaceb, Abderahim LU ; Deierborg, Tomas LU ; Genové, Guillem and Paul, Gesine LU (2018) In Stroke 49(9). p.2182-2190
Abstract

Background and Purpose- In ischemic stroke, breakdown of the blood-brain barrier (BBB) aggravates brain damage. Pericyte detachment contributes to BBB disruption and neurovascular dysfunction, but little is known about its regulation in stroke. Here, we investigated how loss of RGS5 (regulator of G protein signaling 5) in pericytes affects BBB breakdown in stroke and its consequences. Method- We used RGS5 knockout and control mice and applied a permanent middle cerebral occlusion model. We analyzed pericyte numbers, phenotype, and vessel morphology using immunohistochemistry and confocal microscopy. We investigated BBB breakdown by measuring endothelial coverage, tight junctions, and AQP4 (aquaporin 4) in addition to BBB permeability... (More)

Background and Purpose- In ischemic stroke, breakdown of the blood-brain barrier (BBB) aggravates brain damage. Pericyte detachment contributes to BBB disruption and neurovascular dysfunction, but little is known about its regulation in stroke. Here, we investigated how loss of RGS5 (regulator of G protein signaling 5) in pericytes affects BBB breakdown in stroke and its consequences. Method- We used RGS5 knockout and control mice and applied a permanent middle cerebral occlusion model. We analyzed pericyte numbers, phenotype, and vessel morphology using immunohistochemistry and confocal microscopy. We investigated BBB breakdown by measuring endothelial coverage, tight junctions, and AQP4 (aquaporin 4) in addition to BBB permeability (fluorescent-conjugated dextran extravasation). Tissue hypoxia was assessed with pimonidazole hydrochloride and neuronal death quantified with the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Results- We demonstrate that loss of RGS5 increases pericyte numbers and their endothelial coverage, which is associated with higher capillary density and length, and significantly less BBB damage after stroke. Loss of RGS5 in pericytes results in reduced vascular leakage and preserved tight junctions and AQP4, decreased cerebral hypoxia, and partial neuronal protection in the infarct area. Conclusions- Our findings show that loss of RGS5 affects pericyte-related BBB preservation in stroke and identifies RGS5 as an important target for neurovascular protection.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aquaporin 4, blood-brain barrier, ischemia, neuroprotection, pericytes
in
Stroke
volume
49
issue
9
pages
9 pages
publisher
American Heart Association
external identifiers
  • scopus:85055597151
  • pmid:30354999
ISSN
1524-4628
DOI
10.1161/STROKEAHA.118.020124
language
English
LU publication?
yes
id
23c4edf1-3e0d-42c0-92d6-8ae1812f5a73
date added to LUP
2018-11-16 13:06:35
date last changed
2024-06-25 00:31:07
@article{23c4edf1-3e0d-42c0-92d6-8ae1812f5a73,
  abstract     = {{<p>Background and Purpose- In ischemic stroke, breakdown of the blood-brain barrier (BBB) aggravates brain damage. Pericyte detachment contributes to BBB disruption and neurovascular dysfunction, but little is known about its regulation in stroke. Here, we investigated how loss of RGS5 (regulator of G protein signaling 5) in pericytes affects BBB breakdown in stroke and its consequences. Method- We used RGS5 knockout and control mice and applied a permanent middle cerebral occlusion model. We analyzed pericyte numbers, phenotype, and vessel morphology using immunohistochemistry and confocal microscopy. We investigated BBB breakdown by measuring endothelial coverage, tight junctions, and AQP4 (aquaporin 4) in addition to BBB permeability (fluorescent-conjugated dextran extravasation). Tissue hypoxia was assessed with pimonidazole hydrochloride and neuronal death quantified with the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Results- We demonstrate that loss of RGS5 increases pericyte numbers and their endothelial coverage, which is associated with higher capillary density and length, and significantly less BBB damage after stroke. Loss of RGS5 in pericytes results in reduced vascular leakage and preserved tight junctions and AQP4, decreased cerebral hypoxia, and partial neuronal protection in the infarct area. Conclusions- Our findings show that loss of RGS5 affects pericyte-related BBB preservation in stroke and identifies RGS5 as an important target for neurovascular protection.</p>}},
  author       = {{Özen, Ilknur and Roth, Michaela and Barbariga, Marco and Gaceb, Abderahim and Deierborg, Tomas and Genové, Guillem and Paul, Gesine}},
  issn         = {{1524-4628}},
  keywords     = {{aquaporin 4; blood-brain barrier; ischemia; neuroprotection; pericytes}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{2182--2190}},
  publisher    = {{American Heart Association}},
  series       = {{Stroke}},
  title        = {{Loss of Regulator of G-Protein Signaling 5 Leads to Neurovascular Protection in Stroke}},
  url          = {{http://dx.doi.org/10.1161/STROKEAHA.118.020124}},
  doi          = {{10.1161/STROKEAHA.118.020124}},
  volume       = {{49}},
  year         = {{2018}},
}