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Binding of Mucin to Water-Soluble and Surface-Grafted Boronate-Containing Polymers

Ivanov, A. E.; Solodukhina, N. M.; Nilsson, Lars LU ; Nikitin, M. P.; Nikitin, P. I.; Zubov, V. P. and Vikhrov, A. A. (2012) In Polymer Science 54(1). p.1-10
Abstract
The binding of mucin to water-soluble copolymers of N,N-dimethylacrylamide and N-acryloyl-m-aminophenylboronic acid grafted on the surface of glass is studied. Atomic force microscopy studies show that many graft copolymer islands 20-200 nm in diameter and 50 nm in height occur on the modified surface of flat glass. Owing to the presence of phenyl boronate groups, the copolymer behaves as a weak polyelectrolyte (pK(a) = 9.0) and, in the grafted state in an aqueous solution, experiences reversible transitions between states with higher and lower degrees of ionization. As evidenced by spectral correlation interferometry, this phenomenon brings about a change in the thickness of the grafted layer by approximately 0.5 nm. The ability of phenyl... (More)
The binding of mucin to water-soluble copolymers of N,N-dimethylacrylamide and N-acryloyl-m-aminophenylboronic acid grafted on the surface of glass is studied. Atomic force microscopy studies show that many graft copolymer islands 20-200 nm in diameter and 50 nm in height occur on the modified surface of flat glass. Owing to the presence of phenyl boronate groups, the copolymer behaves as a weak polyelectrolyte (pK(a) = 9.0) and, in the grafted state in an aqueous solution, experiences reversible transitions between states with higher and lower degrees of ionization. As evidenced by spectral correlation interferometry, this phenomenon brings about a change in the thickness of the grafted layer by approximately 0.5 nm. The ability of phenyl boronate groups to form cyclic esters with diol and polyol groups results in complexation of the soluble copolymer with mucin oligosaccharides and entails the appearance of slowly growing submicron particles formed by similarly charged polymers. The specificity of complexation is confirmed by dissolution of particles in the presence of fructose: a saccharide with a strong affinity for phenyl boronate groups. The binding of mucin to glass, which is chemically modified with the above copolymer, leads to formation of an adsorption layer with a thickness of 1.2-1.8 nm. Thus, boronate-containing copolymers are suitable for preparing carriers with controllable adsorption properties with respect to polyols, including mucinlike proteins of cellular glycocalyxes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Polymer Science
volume
54
issue
1
pages
1 - 10
publisher
MAIK Nauka/Interperiodica
external identifiers
  • wos:000300282200001
  • scopus:84862106807
ISSN
0965-545X
DOI
10.1134/S0965545X12010026
language
English
LU publication?
yes
id
7bed6bdd-f3db-4adf-964b-7bf34724276c (old id 2409622)
date added to LUP
2012-03-28 12:37:32
date last changed
2017-04-16 03:47:55
@article{7bed6bdd-f3db-4adf-964b-7bf34724276c,
  abstract     = {The binding of mucin to water-soluble copolymers of N,N-dimethylacrylamide and N-acryloyl-m-aminophenylboronic acid grafted on the surface of glass is studied. Atomic force microscopy studies show that many graft copolymer islands 20-200 nm in diameter and 50 nm in height occur on the modified surface of flat glass. Owing to the presence of phenyl boronate groups, the copolymer behaves as a weak polyelectrolyte (pK(a) = 9.0) and, in the grafted state in an aqueous solution, experiences reversible transitions between states with higher and lower degrees of ionization. As evidenced by spectral correlation interferometry, this phenomenon brings about a change in the thickness of the grafted layer by approximately 0.5 nm. The ability of phenyl boronate groups to form cyclic esters with diol and polyol groups results in complexation of the soluble copolymer with mucin oligosaccharides and entails the appearance of slowly growing submicron particles formed by similarly charged polymers. The specificity of complexation is confirmed by dissolution of particles in the presence of fructose: a saccharide with a strong affinity for phenyl boronate groups. The binding of mucin to glass, which is chemically modified with the above copolymer, leads to formation of an adsorption layer with a thickness of 1.2-1.8 nm. Thus, boronate-containing copolymers are suitable for preparing carriers with controllable adsorption properties with respect to polyols, including mucinlike proteins of cellular glycocalyxes.},
  author       = {Ivanov, A. E. and Solodukhina, N. M. and Nilsson, Lars and Nikitin, M. P. and Nikitin, P. I. and Zubov, V. P. and Vikhrov, A. A.},
  issn         = {0965-545X},
  language     = {eng},
  number       = {1},
  pages        = {1--10},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Polymer Science},
  title        = {Binding of Mucin to Water-Soluble and Surface-Grafted Boronate-Containing Polymers},
  url          = {http://dx.doi.org/10.1134/S0965545X12010026},
  volume       = {54},
  year         = {2012},
}