Curcumin decreases acid sphingomyelinase activity in colon cancer caco-2 cells
Cheng, Yajun LU ; Kozubek, Agnieszka ; Ohlsson, Lena LU
; Sternby, Berit
LU
and Duan, Rui-Dong
LU
(2007)
In Planta Medica
73(8).
p.725-730
- Abstract
- Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular... (More)
- Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular sphingomyelin in the cells. Analysis of the hydrolytic products revealed that the activity was derived from acid sphingomyelinase not from phospholipase D. The curcumin-induced reduction of acid SMase required more than 8 h stimulation. Western blotting showed reduced acid sphingomyelinase protein after curcumin stimulation. The inhibitory effect was more potent in monolayer cells than in polarised cells. No changes of other sphingomyelinases were identified. In the concentrations inhibiting acid sphingomyelinase, curcumin inhibited DNA synthesis and induced cell death. In conclusion, curcumin inhibits acid sphingomyelinase and the effect might be involved in its anti proliferative property against colon cancer cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/656986
- author
- Cheng, Yajun
LU
; Kozubek, Agnieszka
; Ohlsson, Lena
LU
; Sternby, Berit
LU
and Duan, Rui-Dong
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell proliferation, curcumin, sphingomyelinase, colon cancer
- in
- Planta Medica
- volume
- 73
- issue
- 8
- pages
- 725 - 730
- publisher
- Georg Thieme Verlag
- external identifiers
-
- wos:000248437500003
- scopus:34547818199
- ISSN
- 0032-0943
- DOI
- 10.1055/s-2007-981540
- language
- English
- LU publication?
- yes
- id
- 2435a289-2aaa-4871-ab77-7bbef6da2a1a (old id 656986)
- date added to LUP
- 2016-04-01 11:35:08
- date last changed
- 2025-04-04 14:33:02
@article{2435a289-2aaa-4871-ab77-7bbef6da2a1a,
abstract = {{Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular sphingomyelin in the cells. Analysis of the hydrolytic products revealed that the activity was derived from acid sphingomyelinase not from phospholipase D. The curcumin-induced reduction of acid SMase required more than 8 h stimulation. Western blotting showed reduced acid sphingomyelinase protein after curcumin stimulation. The inhibitory effect was more potent in monolayer cells than in polarised cells. No changes of other sphingomyelinases were identified. In the concentrations inhibiting acid sphingomyelinase, curcumin inhibited DNA synthesis and induced cell death. In conclusion, curcumin inhibits acid sphingomyelinase and the effect might be involved in its anti proliferative property against colon cancer cells.}},
author = {{Cheng, Yajun and Kozubek, Agnieszka and Ohlsson, Lena and Sternby, Berit and Duan, Rui-Dong}},
issn = {{0032-0943}},
keywords = {{cell proliferation; curcumin; sphingomyelinase; colon cancer}},
language = {{eng}},
number = {{8}},
pages = {{725--730}},
publisher = {{Georg Thieme Verlag}},
series = {{Planta Medica}},
title = {{Curcumin decreases acid sphingomyelinase activity in colon cancer caco-2 cells}},
url = {{http://dx.doi.org/10.1055/s-2007-981540}},
doi = {{10.1055/s-2007-981540}},
volume = {{73}},
year = {{2007}},
}