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Fluorescence spectra provide information on the depth of fluorescent lesions in tissue

Swartling, Johannes LU ; Svensson, Jenny LU ; Bengtsson, D; Terike, K and Andersson-Engels, Stefan LU (2005) In Applied Optics 44(10). p.1934-1941
Abstract
The fluorescence spectrum measured from a fluorophore in tissue is affected by the absorption and scattering properties of the tissue, as well as by the measurement geometry. We analyze this effect with Monte Carlo simulations and by measurements on phantoms. The spectral changes can be used to estimate the depth of a fluorescent lesion embedded in the tissue by measurement of the fluorescence signal in different wavelength bands. By taking the ratio between the signals at two wavelengths, we show that it is possible to determine the depth of the lesion. Simulations were performed and validated by measurements on a phantom in the wavelength range 815-930 nm. The depth of a fluorescing layer could be determined with 0.6-mm accuracy down to... (More)
The fluorescence spectrum measured from a fluorophore in tissue is affected by the absorption and scattering properties of the tissue, as well as by the measurement geometry. We analyze this effect with Monte Carlo simulations and by measurements on phantoms. The spectral changes can be used to estimate the depth of a fluorescent lesion embedded in the tissue by measurement of the fluorescence signal in different wavelength bands. By taking the ratio between the signals at two wavelengths, we show that it is possible to determine the depth of the lesion. Simulations were performed and validated by measurements on a phantom in the wavelength range 815-930 nm. The depth of a fluorescing layer could be determined with 0.6-mm accuracy down to at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types of various composition. The results indicate that depth estimation of a lesion should be possible with 2-3-mm accuracy, with no assumptions made about the optical properties, for a wide range of tissues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Optics
volume
44
issue
10
pages
1934 - 1941
publisher
OSA
external identifiers
  • pmid:15813529
  • wos:000228036600020
  • scopus:16644388497
ISSN
2155-3165
language
English
LU publication?
yes
id
43cd0d45-f030-4483-9308-4d35834cf910 (old id 247238)
alternative location
http://ao.osa.org/abstract.cfm?id=83184
date added to LUP
2007-08-13 16:29:30
date last changed
2017-11-05 03:35:29
@article{43cd0d45-f030-4483-9308-4d35834cf910,
  abstract     = {The fluorescence spectrum measured from a fluorophore in tissue is affected by the absorption and scattering properties of the tissue, as well as by the measurement geometry. We analyze this effect with Monte Carlo simulations and by measurements on phantoms. The spectral changes can be used to estimate the depth of a fluorescent lesion embedded in the tissue by measurement of the fluorescence signal in different wavelength bands. By taking the ratio between the signals at two wavelengths, we show that it is possible to determine the depth of the lesion. Simulations were performed and validated by measurements on a phantom in the wavelength range 815-930 nm. The depth of a fluorescing layer could be determined with 0.6-mm accuracy down to at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types of various composition. The results indicate that depth estimation of a lesion should be possible with 2-3-mm accuracy, with no assumptions made about the optical properties, for a wide range of tissues.},
  author       = {Swartling, Johannes and Svensson, Jenny and Bengtsson, D and Terike, K and Andersson-Engels, Stefan},
  issn         = {2155-3165},
  language     = {eng},
  number       = {10},
  pages        = {1934--1941},
  publisher    = {OSA},
  series       = {Applied Optics},
  title        = {Fluorescence spectra provide information on the depth of fluorescent lesions in tissue},
  volume       = {44},
  year         = {2005},
}