Fluorescence spectra provide information on the depth of fluorescent lesions in tissue
(2005) In Applied Optics 44(10). p.1934-1941- Abstract
- The fluorescence spectrum measured from a fluorophore in tissue is affected by the absorption and scattering properties of the tissue, as well as by the measurement geometry. We analyze this effect with Monte Carlo simulations and by measurements on phantoms. The spectral changes can be used to estimate the depth of a fluorescent lesion embedded in the tissue by measurement of the fluorescence signal in different wavelength bands. By taking the ratio between the signals at two wavelengths, we show that it is possible to determine the depth of the lesion. Simulations were performed and validated by measurements on a phantom in the wavelength range 815-930 nm. The depth of a fluorescing layer could be determined with 0.6-mm accuracy down to... (More)
- The fluorescence spectrum measured from a fluorophore in tissue is affected by the absorption and scattering properties of the tissue, as well as by the measurement geometry. We analyze this effect with Monte Carlo simulations and by measurements on phantoms. The spectral changes can be used to estimate the depth of a fluorescent lesion embedded in the tissue by measurement of the fluorescence signal in different wavelength bands. By taking the ratio between the signals at two wavelengths, we show that it is possible to determine the depth of the lesion. Simulations were performed and validated by measurements on a phantom in the wavelength range 815-930 nm. The depth of a fluorescing layer could be determined with 0.6-mm accuracy down to at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types of various composition. The results indicate that depth estimation of a lesion should be possible with 2-3-mm accuracy, with no assumptions made about the optical properties, for a wide range of tissues. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/247238
- author
- Swartling, Johannes LU ; Svensson, Jenny LU ; Bengtsson, D ; Terike, K and Andersson-Engels, Stefan LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Optics
- volume
- 44
- issue
- 10
- pages
- 1934 - 1941
- publisher
- Optical Society of America
- external identifiers
-
- pmid:15813529
- wos:000228036600020
- scopus:16644388497
- ISSN
- 2155-3165
- language
- English
- LU publication?
- yes
- id
- 43cd0d45-f030-4483-9308-4d35834cf910 (old id 247238)
- alternative location
- http://ao.osa.org/abstract.cfm?id=83184
- date added to LUP
- 2016-04-01 11:56:29
- date last changed
- 2022-04-21 00:03:37
@article{43cd0d45-f030-4483-9308-4d35834cf910,
abstract = {{The fluorescence spectrum measured from a fluorophore in tissue is affected by the absorption and scattering properties of the tissue, as well as by the measurement geometry. We analyze this effect with Monte Carlo simulations and by measurements on phantoms. The spectral changes can be used to estimate the depth of a fluorescent lesion embedded in the tissue by measurement of the fluorescence signal in different wavelength bands. By taking the ratio between the signals at two wavelengths, we show that it is possible to determine the depth of the lesion. Simulations were performed and validated by measurements on a phantom in the wavelength range 815-930 nm. The depth of a fluorescing layer could be determined with 0.6-mm accuracy down to at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types of various composition. The results indicate that depth estimation of a lesion should be possible with 2-3-mm accuracy, with no assumptions made about the optical properties, for a wide range of tissues.}},
author = {{Swartling, Johannes and Svensson, Jenny and Bengtsson, D and Terike, K and Andersson-Engels, Stefan}},
issn = {{2155-3165}},
language = {{eng}},
number = {{10}},
pages = {{1934--1941}},
publisher = {{Optical Society of America}},
series = {{Applied Optics}},
title = {{Fluorescence spectra provide information on the depth of fluorescent lesions in tissue}},
url = {{https://lup.lub.lu.se/search/files/2711949/2370907.pdf}},
volume = {{44}},
year = {{2005}},
}