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Effect of pulsed electric field on the germination of barley seeds

Dymek, Katarzyna LU ; Dejmek, Petr LU ; Panarese, Valentina; Vicente, Antonio A.; Wadsö, Lars LU ; Finnie, Christine and Gomez, Federico LU (2012) In LWT- Food Science and Technology 47(1). p.161-166
Abstract
This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEE). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEE affects radicle emergence without significantly affecting the seeds gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection... (More)
This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEE). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEE affects radicle emergence without significantly affecting the seeds gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that a-amylase concentration decreases in the PEF-treated seeds. (C) 2011 Elsevier Ltd. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Isothermal calorimetry, Radical emergence, alpha-Amylase
in
LWT- Food Science and Technology
volume
47
issue
1
pages
161 - 166
publisher
Elsevier
external identifiers
  • wos:000301750100024
  • scopus:84857110933
ISSN
0023-6438
DOI
10.1016/j.lwt.2011.12.019
language
English
LU publication?
yes
id
8c75cdd7-75fb-4a36-88d9-877702efd25f (old id 2494959)
date added to LUP
2012-05-10 14:01:00
date last changed
2017-07-30 04:00:54
@article{8c75cdd7-75fb-4a36-88d9-877702efd25f,
  abstract     = {This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEE). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEE affects radicle emergence without significantly affecting the seeds gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that a-amylase concentration decreases in the PEF-treated seeds. (C) 2011 Elsevier Ltd. All rights reserved.},
  author       = {Dymek, Katarzyna and Dejmek, Petr and Panarese, Valentina and Vicente, Antonio A. and Wadsö, Lars and Finnie, Christine and Gomez, Federico},
  issn         = {0023-6438},
  keyword      = {Isothermal calorimetry,Radical emergence,alpha-Amylase},
  language     = {eng},
  number       = {1},
  pages        = {161--166},
  publisher    = {Elsevier},
  series       = {LWT- Food Science and Technology},
  title        = {Effect of pulsed electric field on the germination of barley seeds},
  url          = {http://dx.doi.org/10.1016/j.lwt.2011.12.019},
  volume       = {47},
  year         = {2012},
}