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Amyloid-beta Oligomers in Cerebrospinal Fluid are Associated with Cognitive Decline in Patients with Alzheimer's Disease

Santos, Alexander Navarrete; Ewers, Michael; Minthon, Lennart LU ; Simm, Andreas; Silber, Rolf-Edgar; Blennow, Kaj; Prvulovic, David; Hansson, Oskar LU and Hampel, Harald (2012) In Journal of Alzheimer's Disease 29(1). p.171-176
Abstract
Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer... (More)
Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio A beta-oligomers/A beta(42) was significantly elevated in AD subjects compared to non-AD subjects (p=0.001). Most important, there was a negative correlation between the amount of A beta-oligomers and the Mini-Mental Status Exam score (r=-0.65; p=0.013) in AD patients. The detection of A beta-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oligomers, cytometry, flow, cognitive decline, biomarkers, amyloid-beta, Alzheimer's disease
in
Journal of Alzheimer's Disease
volume
29
issue
1
pages
171 - 176
publisher
IOS Press
external identifiers
  • wos:000301139000015
ISSN
1387-2877
DOI
10.3233/JAD-2011-111361
language
English
LU publication?
yes
id
bc13f555-1628-40cc-a4bf-2efbbb1c7cdd (old id 2517551)
date added to LUP
2012-05-07 07:28:05
date last changed
2016-04-15 14:37:53
@article{bc13f555-1628-40cc-a4bf-2efbbb1c7cdd,
  abstract     = {Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio A beta-oligomers/A beta(42) was significantly elevated in AD subjects compared to non-AD subjects (p=0.001). Most important, there was a negative correlation between the amount of A beta-oligomers and the Mini-Mental Status Exam score (r=-0.65; p=0.013) in AD patients. The detection of A beta-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity.},
  author       = {Santos, Alexander Navarrete and Ewers, Michael and Minthon, Lennart and Simm, Andreas and Silber, Rolf-Edgar and Blennow, Kaj and Prvulovic, David and Hansson, Oskar and Hampel, Harald},
  issn         = {1387-2877},
  keyword      = {oligomers,cytometry,flow,cognitive decline,biomarkers,amyloid-beta,Alzheimer's disease},
  language     = {eng},
  number       = {1},
  pages        = {171--176},
  publisher    = {IOS Press},
  series       = {Journal of Alzheimer's Disease},
  title        = {Amyloid-beta Oligomers in Cerebrospinal Fluid are Associated with Cognitive Decline in Patients with Alzheimer's Disease},
  url          = {http://dx.doi.org/10.3233/JAD-2011-111361},
  volume       = {29},
  year         = {2012},
}