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The adult human brain harbors multipotent perivascular mesenchymal stem cells.

Paul-Visse, Gesine LU ; Ozen, Ilknur LU ; Christophersen, Nicolaj; Reinbothe, Thomas LU ; Bengzon, Johan LU ; Visse, Edward LU ; Jansson, Katarina; Dannaeus, Karin; Henriques-Oliveira, Catarina and Roybon, Laurent LU , et al. (2012) In PLoS ONE 7(4).
Abstract
Blood vessels and adjacent cells form perivascular stem cell niches in adult tissues. In this perivascular niche, a stem cell with mesenchymal characteristics was recently identified in some adult somatic tissues. These cells are pericytes that line the microvasculature, express mesenchymal markers and differentiate into mesodermal lineages but might even have the capacity to generate tissue-specific cell types. Here, we isolated, purified and characterized a previously unrecognized progenitor population from two different regions in the adult human brain, the ventricular wall and the neocortex. We show that these cells co-express markers for mesenchymal stem cells and pericytes in vivo and in vitro, but do not express glial, neuronal... (More)
Blood vessels and adjacent cells form perivascular stem cell niches in adult tissues. In this perivascular niche, a stem cell with mesenchymal characteristics was recently identified in some adult somatic tissues. These cells are pericytes that line the microvasculature, express mesenchymal markers and differentiate into mesodermal lineages but might even have the capacity to generate tissue-specific cell types. Here, we isolated, purified and characterized a previously unrecognized progenitor population from two different regions in the adult human brain, the ventricular wall and the neocortex. We show that these cells co-express markers for mesenchymal stem cells and pericytes in vivo and in vitro, but do not express glial, neuronal progenitor, hematopoietic, endothelial or microglial markers in their native state. Furthermore, we demonstrate at a clonal level that these progenitors have true multilineage potential towards both, the mesodermal and neuroectodermal phenotype. They can be epigenetically induced in vitro into adipocytes, chondroblasts and osteoblasts but also into glial cells and immature neurons. This progenitor population exhibits long-term proliferation, karyotype stability and retention of phenotype and multipotency following extensive propagation. Thus, we provide evidence that the vascular niche in the adult human brain harbors a novel progenitor with multilineage capacity that appears to represent mesenchymal stem cells and is different from any previously described human neural stem cell. Future studies will elucidate whether these cells may play a role for disease or may represent a reservoir that can be exploited in efforts to repair the diseased human brain. (Less)
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PLoS ONE
volume
7
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4
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Public Library of Science
external identifiers
  • wos:000305345000076
  • pmid:22523602
  • scopus:84859865368
ISSN
1932-6203
DOI
10.1371/journal.pone.0035577
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English
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yes
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aa7d3037-1b9c-4f2e-aa09-e521898a66be (old id 2519187)
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http://www.ncbi.nlm.nih.gov/pubmed/22523602?dopt=Abstract
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2012-05-07 11:26:13
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@article{aa7d3037-1b9c-4f2e-aa09-e521898a66be,
  abstract     = {Blood vessels and adjacent cells form perivascular stem cell niches in adult tissues. In this perivascular niche, a stem cell with mesenchymal characteristics was recently identified in some adult somatic tissues. These cells are pericytes that line the microvasculature, express mesenchymal markers and differentiate into mesodermal lineages but might even have the capacity to generate tissue-specific cell types. Here, we isolated, purified and characterized a previously unrecognized progenitor population from two different regions in the adult human brain, the ventricular wall and the neocortex. We show that these cells co-express markers for mesenchymal stem cells and pericytes in vivo and in vitro, but do not express glial, neuronal progenitor, hematopoietic, endothelial or microglial markers in their native state. Furthermore, we demonstrate at a clonal level that these progenitors have true multilineage potential towards both, the mesodermal and neuroectodermal phenotype. They can be epigenetically induced in vitro into adipocytes, chondroblasts and osteoblasts but also into glial cells and immature neurons. This progenitor population exhibits long-term proliferation, karyotype stability and retention of phenotype and multipotency following extensive propagation. Thus, we provide evidence that the vascular niche in the adult human brain harbors a novel progenitor with multilineage capacity that appears to represent mesenchymal stem cells and is different from any previously described human neural stem cell. Future studies will elucidate whether these cells may play a role for disease or may represent a reservoir that can be exploited in efforts to repair the diseased human brain.},
  articleno    = {e35577},
  author       = {Paul-Visse, Gesine and Ozen, Ilknur and Christophersen, Nicolaj and Reinbothe, Thomas and Bengzon, Johan and Visse, Edward and Jansson, Katarina and Dannaeus, Karin and Henriques-Oliveira, Catarina and Roybon, Laurent and Anisimov, Sergey and Renström, Erik and Svensson, Mikael and Haegerstrand, Anders and Brundin, Patrik},
  issn         = {1932-6203},
  language     = {eng},
  number       = {4},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {The adult human brain harbors multipotent perivascular mesenchymal stem cells.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0035577},
  volume       = {7},
  year         = {2012},
}