Adaptor Protein LNK Is a Negative Regulator of Brain Neural Stem Cell Proliferation after Stroke.
(2012) In The Journal of Neuroscience : the official journal of the Society for Neuroscience 32(15). p.5151-5164- Abstract
- Ischemic stroke causes transient increase of neural stem and progenitor cell (NSPC) proliferation in the subventricular zone (SVZ), and migration of newly formed neuroblasts toward the damaged area where they mature to striatal neurons. The molecular mechanisms regulating this plastic response, probably involved in structural reorganization and functional recovery, are poorly understood. The adaptor protein LNK suppresses hematopoietic stem cell self-renewal, but its presence and role in the brain are poorly understood. Here we demonstrate that LNK is expressed in NSPCs in the adult mouse and human SVZ. Lnk(-/-) mice exhibited increased NSPC proliferation after stroke, but not in intact brain or following status epilepticus. Deletion of... (More)
- Ischemic stroke causes transient increase of neural stem and progenitor cell (NSPC) proliferation in the subventricular zone (SVZ), and migration of newly formed neuroblasts toward the damaged area where they mature to striatal neurons. The molecular mechanisms regulating this plastic response, probably involved in structural reorganization and functional recovery, are poorly understood. The adaptor protein LNK suppresses hematopoietic stem cell self-renewal, but its presence and role in the brain are poorly understood. Here we demonstrate that LNK is expressed in NSPCs in the adult mouse and human SVZ. Lnk(-/-) mice exhibited increased NSPC proliferation after stroke, but not in intact brain or following status epilepticus. Deletion of Lnk caused increased NSPC proliferation while overexpression decreased mitotic activity of these cells in vitro. We found that Lnk expression after stroke increased in SVZ through the transcription factors STAT1/3. LNK attenuated insulin-like growth factor 1 signaling by inhibition of AKT phosphorylation, resulting in reduced NSPC proliferation. Our findings identify LNK as a stroke-specific, endogenous negative regulator of NSPC proliferation, and suggest that LNK signaling is a novel mechanism influencing plastic responses in postischemic brain. (Less)
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https://lup.lub.lu.se/record/2519520
- author
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Neuroscience : the official journal of the Society for Neuroscience
- volume
- 32
- issue
- 15
- pages
- 5151 - 5164
- publisher
- Society for Neuroscience
- external identifiers
-
- wos:000302793500014
- pmid:22496561
- scopus:84859520686
- ISSN
- 1529-2401
- DOI
- 10.1523/JNEUROSCI.0474-12.2012
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Pathology, (Lund) (013030000), Stem Cell Center (013041110), Developmental Neurobiology (013210001)
- id
- f1f365a4-78fe-495b-b757-eb16098af283 (old id 2519520)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22496561?dopt=Abstract
- date added to LUP
- 2016-04-04 09:10:08
- date last changed
- 2024-08-03 13:28:20
@article{f1f365a4-78fe-495b-b757-eb16098af283, abstract = {{Ischemic stroke causes transient increase of neural stem and progenitor cell (NSPC) proliferation in the subventricular zone (SVZ), and migration of newly formed neuroblasts toward the damaged area where they mature to striatal neurons. The molecular mechanisms regulating this plastic response, probably involved in structural reorganization and functional recovery, are poorly understood. The adaptor protein LNK suppresses hematopoietic stem cell self-renewal, but its presence and role in the brain are poorly understood. Here we demonstrate that LNK is expressed in NSPCs in the adult mouse and human SVZ. Lnk(-/-) mice exhibited increased NSPC proliferation after stroke, but not in intact brain or following status epilepticus. Deletion of Lnk caused increased NSPC proliferation while overexpression decreased mitotic activity of these cells in vitro. We found that Lnk expression after stroke increased in SVZ through the transcription factors STAT1/3. LNK attenuated insulin-like growth factor 1 signaling by inhibition of AKT phosphorylation, resulting in reduced NSPC proliferation. Our findings identify LNK as a stroke-specific, endogenous negative regulator of NSPC proliferation, and suggest that LNK signaling is a novel mechanism influencing plastic responses in postischemic brain.}}, author = {{Ahlenius, Henrik and Devaraju, Karthikeyan and Monni, Emanuela and Oki, Koichi and Wattananit, Somsak and Darsalia, Vladimer and Iosif, Robert and Torper, Olof and Wood, James and Braun, Sebastian and Jagemann, Lucas and Nuber, Ulrike and Englund, Elisabet and Jacobsen, Sten Eirik W and Lindvall, Olle and Kokaia, Zaal}}, issn = {{1529-2401}}, language = {{eng}}, number = {{15}}, pages = {{5151--5164}}, publisher = {{Society for Neuroscience}}, series = {{The Journal of Neuroscience : the official journal of the Society for Neuroscience}}, title = {{Adaptor Protein LNK Is a Negative Regulator of Brain Neural Stem Cell Proliferation after Stroke.}}, url = {{http://dx.doi.org/10.1523/JNEUROSCI.0474-12.2012}}, doi = {{10.1523/JNEUROSCI.0474-12.2012}}, volume = {{32}}, year = {{2012}}, }