Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Glucuronosylated and linear xylooligosaccharides from Quinoa stalks xylan as potential prebiotic source for growth of Bifidobacterium adolescentis and Weissella cibaria

Salas-Veizaga, Daniel Martin LU ; Bhattacharya, Abhishek ; Adlercreutz, Patrick LU orcid ; Stålbrand, Henrik LU and Nordberg Karlsson, Eva LU orcid (2021) In LWT - Food Science and Technology 152.
Abstract
Quinoa stalks glucuronoarabinoxylan (QSGAX) has been extracted by
alkali and further utilized for production of two-types of
xylooligosaccharides (XOs): i) enzymatically produced
glucuronosylated-XOs (GXOs), and ii) a dilute acid produced mixture of
non-substituted/substituted XOs of different degree of polymerization
(DP). The respective mixtures were then separately evaluated as prebiotics by analysis of their consumption by two phylogenetically different potential probiotic bacterial strains (Bifidobacterium adolescentis ATCC15703 and Weissella cibaria strain 92), from which release of short chain fatty acids (SCFA) was also monitored. The GXOs mixture was produced using a... (More)
Quinoa stalks glucuronoarabinoxylan (QSGAX) has been extracted by
alkali and further utilized for production of two-types of
xylooligosaccharides (XOs): i) enzymatically produced
glucuronosylated-XOs (GXOs), and ii) a dilute acid produced mixture of
non-substituted/substituted XOs of different degree of polymerization
(DP). The respective mixtures were then separately evaluated as prebiotics by analysis of their consumption by two phylogenetically different potential probiotic bacterial strains (Bifidobacterium adolescentis ATCC15703 and Weissella cibaria strain 92), from which release of short chain fatty acids (SCFA) was also monitored. The GXOs mixture was produced using a
glucuronosyl-requiring family 30 glycoside hydrolase (GH) Bacteroides ovatus (BoXyn30A), while the XOs mixture was produced by a 30 min acid treatment of QSGAX with H2SO4 [24.5 g/L] at 90 °C. B. adolescentis
consumed both GXOs and XOs (DP 2–6), in both cases releasing acetate,
lactate, propionate, formate and butyrate as metabolic products. W. cibaria
only consumed XOs (DP 2–4), releasing acetate, lactate and minor
amounts of butyrate. This is the first study reporting the ability of
GXOs consumption by B. adolescentis and shows the potential of GXOs to selectively stimulate B. adolescentis, while XOs stimulated both types of potential probiotics (B. adolescentis ATCC15703 and W. cibaria strain 92). (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Quinoa stalks, Glucuronoarabinoxylan, Glucurono-xylooligosaccharides, Bifidobacterium adolescentis ATCC15703, Weissella cibaria strain 92
in
LWT - Food Science and Technology
volume
152
article number
112348
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85114193700
ISSN
0023-6438
DOI
10.1016/j.lwt.2021.112348
language
English
LU publication?
yes
id
25235a11-7243-405e-b56c-196818896d40
date added to LUP
2021-09-07 14:48:14
date last changed
2022-04-27 03:42:27
@article{25235a11-7243-405e-b56c-196818896d40,
  abstract     = {{Quinoa stalks glucuronoarabinoxylan (QSGAX) has been extracted by <br/>alkali and further utilized for production of two-types of <br/>xylooligosaccharides (XOs): i) enzymatically produced <br/>glucuronosylated-XOs (GXOs), and ii) a dilute acid produced mixture of <br/>non-substituted/substituted XOs of different degree of polymerization <br/>(DP). The respective mixtures were then separately evaluated as prebiotics by analysis of their consumption by two phylogenetically different potential probiotic bacterial strains (<i>Bifidobacterium adolescentis</i> ATCC15703 and <i>Weissella cibaria </i>strain 92), from which release of short chain fatty acids (SCFA) was also monitored. The GXOs mixture was produced using a <br/>glucuronosyl-requiring family 30 glycoside hydrolase (GH) Bacteroides ovatus (<i>Bo</i>Xyn30A), while the XOs mixture was produced by a 30 min acid treatment of QSGAX with H<sub>2</sub>SO<sub>4</sub> [24.5 g/L] at 90 °C. <i>B. adolescentis</i><br/> consumed both GXOs and XOs (DP 2–6), in both cases releasing acetate, <br/>lactate, propionate, formate and butyrate as metabolic products. <i>W. cibaria</i><br/> only consumed XOs (DP 2–4), releasing acetate, lactate and minor <br/>amounts of butyrate. This is the first study reporting the ability of <br/>GXOs consumption by <i>B. adolescentis</i> and shows the potential of GXOs to selectively stimulate <i>B. adolescentis</i>, while XOs stimulated both types of potential probiotics (<i>B. adolescentis</i> ATCC15703 and <i>W. cibaria</i> strain 92).}},
  author       = {{Salas-Veizaga, Daniel Martin and Bhattacharya, Abhishek and Adlercreutz, Patrick and Stålbrand, Henrik and Nordberg Karlsson, Eva}},
  issn         = {{0023-6438}},
  keywords     = {{Quinoa stalks; Glucuronoarabinoxylan; Glucurono-xylooligosaccharides; Bifidobacterium adolescentis ATCC15703; Weissella cibaria strain 92}},
  language     = {{eng}},
  month        = {{08}},
  publisher    = {{Elsevier}},
  series       = {{LWT - Food Science and Technology}},
  title        = {{Glucuronosylated and linear xylooligosaccharides from Quinoa stalks xylan as potential prebiotic source for growth of <i>Bifidobacterium adolescentis</i> and <i>Weissella cibaria</i>}},
  url          = {{http://dx.doi.org/10.1016/j.lwt.2021.112348}},
  doi          = {{10.1016/j.lwt.2021.112348}},
  volume       = {{152}},
  year         = {{2021}},
}