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Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems

Gvazava, Nika LU ; Konings, Sabine LU orcid ; Cepeda-Prado, Efrain LU ; Skoryk, Valeriia LU ; Umeano, Chimezie H. LU ; Dong, Jiao LU orcid ; Silva, Iran A.N. LU orcid ; Ottosson, Daniella Rylander LU orcid ; Leigh, Nicholas D. LU orcid and Wagner, Darcy E. LU orcid , et al. (2023)
Abstract
Label-free chemical and structural imaging of complex living tissue and biological systems is the holy grail of biomedical research and clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sample preparation, often due to the presence of interfering molecules such as water, making them unsuitable for the analysis of such systems. Here, we demonstrate a proof-of-principle study using label-free optical photothermal mid-infrared microspectroscopy (O-PTIR) for fast, direct spatiotemporal chemical analysis of complex living biological systems at submicron resolution. While other analytical methods can provide only static snapshots of molecular structures, our O-PTIR approach enables time-resolved... (More)
Label-free chemical and structural imaging of complex living tissue and biological systems is the holy grail of biomedical research and clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sample preparation, often due to the presence of interfering molecules such as water, making them unsuitable for the analysis of such systems. Here, we demonstrate a proof-of-principle study using label-free optical photothermal mid-infrared microspectroscopy (O-PTIR) for fast, direct spatiotemporal chemical analysis of complex living biological systems at submicron resolution. While other analytical methods can provide only static snapshots of molecular structures, our O-PTIR approach enables time-resolved and in situ investigation of chemical and structural changes of diverse biomolecules in their native conditions. This comprises a technological breakthrough in infrared spectroscopy to analyze biomolecules under native conditions over time: in fresh unprocessed biopsies, living brain tissue, and vertebrates without compromising their viability. (Less)
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publishing date
type
Working paper/Preprint
publication status
published
subject
publisher
bioRxiv
DOI
10.1101/2023.01.05.522847
language
English
LU publication?
yes
id
2543bf9b-d3f3-40dc-ae9a-450bdea623d9
date added to LUP
2025-10-16 13:44:08
date last changed
2025-10-16 15:07:51
@misc{2543bf9b-d3f3-40dc-ae9a-450bdea623d9,
  abstract     = {{Label-free chemical and structural imaging of complex living tissue and biological systems is the holy grail of biomedical research and clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sample preparation, often due to the presence of interfering molecules such as water, making them unsuitable for the analysis of such systems. Here, we demonstrate a proof-of-principle study using label-free optical photothermal mid-infrared microspectroscopy (O-PTIR) for fast, direct spatiotemporal chemical analysis of complex living biological systems at submicron resolution. While other analytical methods can provide only static snapshots of molecular structures, our O-PTIR approach enables time-resolved and in situ investigation of chemical and structural changes of diverse biomolecules in their native conditions. This comprises a technological breakthrough in infrared spectroscopy to analyze biomolecules under native conditions over time: in fresh unprocessed biopsies, living brain tissue, and vertebrates without compromising their viability.}},
  author       = {{Gvazava, Nika and Konings, Sabine and Cepeda-Prado, Efrain and Skoryk, Valeriia and Umeano, Chimezie H. and Dong, Jiao and Silva, Iran A.N. and Ottosson, Daniella Rylander and Leigh, Nicholas D. and Wagner, Darcy E. and Klementieva, Oxana}},
  language     = {{eng}},
  month        = {{01}},
  note         = {{Preprint}},
  publisher    = {{bioRxiv}},
  title        = {{Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems}},
  url          = {{http://dx.doi.org/10.1101/2023.01.05.522847}},
  doi          = {{10.1101/2023.01.05.522847}},
  year         = {{2023}},
}