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Stability of peptide-HLA-I complexes and tapasin folding facilitation - tools to define immunogenic peptides

Geironson Ulfsson, Linda LU ; Roder, Gustav and Paulsson, Kajsa M LU (2012) In FEBS Letters 586(9). p.1336-1343
Abstract
Only a small fraction of the peptides generated inside the cell end up being presented by HLA-I on the cell surface. High stability of peptide-HLA-I complexes and a low HLA-I tapasin-facilitation have been proposed to predict immunogenicity. We here set out to investigate if these parameters correlated and defined immunogenic peptides. Both peptide-HLA-B*08:01 and peptide-HLA-A*02:01 complexes showed small differences in tapasin-facilitation and larger differences in stability. This suggests that the stability of immunogenic peptide-HLA-I complexes vary above an HLA-I allomorph dependent lower limit (e. g. > 2 h for HLA-A*02:01), immunogenicity predicted by tapasin-facilitation may be defined by an equally allomorph unique upper value... (More)
Only a small fraction of the peptides generated inside the cell end up being presented by HLA-I on the cell surface. High stability of peptide-HLA-I complexes and a low HLA-I tapasin-facilitation have been proposed to predict immunogenicity. We here set out to investigate if these parameters correlated and defined immunogenic peptides. Both peptide-HLA-B*08:01 and peptide-HLA-A*02:01 complexes showed small differences in tapasin-facilitation and larger differences in stability. This suggests that the stability of immunogenic peptide-HLA-I complexes vary above an HLA-I allomorph dependent lower limit (e. g. > 2 h for HLA-A*02:01), immunogenicity predicted by tapasin-facilitation may be defined by an equally allomorph unique upper value (e. g. tapasin-facilitation <1.5 for HLA-A*02:01), and variation above the stability-threshold does not directly reflect a variation in tapasin-facilitation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Tapasin, MHC-I, Peptide, Stability, Vaccine
in
FEBS Letters
volume
586
issue
9
pages
1336 - 1343
publisher
Wiley-Blackwell
external identifiers
  • wos:000303434200014
  • pmid:22616994
  • scopus:84860343019
ISSN
1873-3468
DOI
10.1016/j.febslet.2012.03.045
language
English
LU publication?
yes
id
b5209b6f-1909-4cd6-b5f5-023b7758cc23 (old id 2545441)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22616994?dopt=Abstract
date added to LUP
2012-06-01 08:53:53
date last changed
2017-01-01 06:16:11
@article{b5209b6f-1909-4cd6-b5f5-023b7758cc23,
  abstract     = {Only a small fraction of the peptides generated inside the cell end up being presented by HLA-I on the cell surface. High stability of peptide-HLA-I complexes and a low HLA-I tapasin-facilitation have been proposed to predict immunogenicity. We here set out to investigate if these parameters correlated and defined immunogenic peptides. Both peptide-HLA-B*08:01 and peptide-HLA-A*02:01 complexes showed small differences in tapasin-facilitation and larger differences in stability. This suggests that the stability of immunogenic peptide-HLA-I complexes vary above an HLA-I allomorph dependent lower limit (e. g. &gt; 2 h for HLA-A*02:01), immunogenicity predicted by tapasin-facilitation may be defined by an equally allomorph unique upper value (e. g. tapasin-facilitation &lt;1.5 for HLA-A*02:01), and variation above the stability-threshold does not directly reflect a variation in tapasin-facilitation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.},
  author       = {Geironson Ulfsson, Linda and Roder, Gustav and Paulsson, Kajsa M},
  issn         = {1873-3468},
  keyword      = {Tapasin,MHC-I,Peptide,Stability,Vaccine},
  language     = {eng},
  number       = {9},
  pages        = {1336--1343},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Stability of peptide-HLA-I complexes and tapasin folding facilitation - tools to define immunogenic peptides},
  url          = {http://dx.doi.org/10.1016/j.febslet.2012.03.045},
  volume       = {586},
  year         = {2012},
}