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Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression

Lagergren, Anna LU ; Manetopoulos, Christina LU ; Axelson, Håkan LU and Sigvardsson, Mikael LU (2004) In BMC Cancer 4.
Abstract
Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was... (More)
Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was further investigated by EMSA analysis. Results: In pre-B cells mb-1 and CD19 are regulate by EBF-1 in collaboration with Pax-5 and E-proteins. We here show that neuroblastoma cells express these three, for B cell development crucial transcription factors, but nevertheless fail to express detectable levels of their known target genes. Expression of mb-1 could, however, be induced in neuroblastoma cells after disruption of the chromatin structure by treatment with 5-azacytidine and Trichostatin A. Conclusion: These data suggest that transcription factors are able to selectively activate target genes in different tissues and that chromatin structure plays a key role in the regulation of this activity. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
BMC Cancer
volume
4
publisher
BioMed Central
external identifiers
  • pmid:15544702
  • wos:000226525400001
  • scopus:13144291649
ISSN
1471-2407
DOI
10.1186/1471-2407-4-80
language
English
LU publication?
yes
id
04f52da9-ea47-4f15-9eac-9b7027cc237f (old id 254957)
date added to LUP
2007-10-24 07:34:48
date last changed
2017-01-01 07:19:14
@article{04f52da9-ea47-4f15-9eac-9b7027cc237f,
  abstract     = {Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was further investigated by EMSA analysis. Results: In pre-B cells mb-1 and CD19 are regulate by EBF-1 in collaboration with Pax-5 and E-proteins. We here show that neuroblastoma cells express these three, for B cell development crucial transcription factors, but nevertheless fail to express detectable levels of their known target genes. Expression of mb-1 could, however, be induced in neuroblastoma cells after disruption of the chromatin structure by treatment with 5-azacytidine and Trichostatin A. Conclusion: These data suggest that transcription factors are able to selectively activate target genes in different tissues and that chromatin structure plays a key role in the regulation of this activity.},
  author       = {Lagergren, Anna and Manetopoulos, Christina and Axelson, Håkan and Sigvardsson, Mikael},
  issn         = {1471-2407},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Cancer},
  title        = {Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression},
  url          = {http://dx.doi.org/10.1186/1471-2407-4-80},
  volume       = {4},
  year         = {2004},
}