Advanced

Helicobacter pylori infection assessed by ELISA and by immunoblot and noncardia gastric cancer risk in a prospective study: the Eurgast-EPIC project

Gonzalez, C. A.; Megraud, F.; Buissonniere, A.; Lujan Barroso, L.; Agudo, A.; Duell, E. J.; Boutron-Ruault, M. C.; Clavel-Chapelon, F.; Palli, D. and Krogh, V., et al. (2012) In Annals of Oncology 23(5). p.1320-1324
Abstract
In epidemiological studies, Helicobacter pylori infection is usually detected by enzyme-linked immunosorbent assay (ELISA). However, infection can spontaneously clear from the mucosa during the progression of atrophy and could lead to substantial under-detection of infection and underestimation of its effect on gastric cancer (GC) risk. Antibodies detected by western blot are known to persist longer after the loss of the infection. In a nested case-control study from the Eurogast-EPIC cohort, including 88 noncardia GC cases and 338 controls, we assessed the association between noncardia GC and H. pylori infection comparing antibodies detected by western blot (HELICOBLOT2.1) to those detected by ELISA (Pyloriset EIA-GIII((R))). By... (More)
In epidemiological studies, Helicobacter pylori infection is usually detected by enzyme-linked immunosorbent assay (ELISA). However, infection can spontaneously clear from the mucosa during the progression of atrophy and could lead to substantial under-detection of infection and underestimation of its effect on gastric cancer (GC) risk. Antibodies detected by western blot are known to persist longer after the loss of the infection. In a nested case-control study from the Eurogast-EPIC cohort, including 88 noncardia GC cases and 338 controls, we assessed the association between noncardia GC and H. pylori infection comparing antibodies detected by western blot (HELICOBLOT2.1) to those detected by ELISA (Pyloriset EIA-GIII((R))). By immunoblot, 82 cases (93.2%) were H. pylori positive, 10 of these cases (11.4%) were negative by ELISA and only 6 cases (6.8%) were negative by both ELISA and immunoblot. Multivariable odds ratio (OR) for noncardia GC comparing immunoglobulin G positive versus negative by ELISA was 6.8 [95% confidence interval (CI) 3.0-15.1], and by immunoblot, the OR was 21.4 (95% CI 7.1-64.4). Using a western blot assay, nearly all noncardia GC were classified as H. pylori positive and the OR was more than threefold higher than the OR assessed by ELISA, supporting the hypothesis that H. pylori infection is a necessary condition for noncardia GC. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Helicobacter pylori, noncardia gastric cancer, prospective study, western blot
in
Annals of Oncology
volume
23
issue
5
pages
1320 - 1324
publisher
Oxford University Press
external identifiers
  • wos:000303336400035
  • scopus:84859403982
ISSN
1569-8041
DOI
10.1093/annonc/mdr384
language
English
LU publication?
yes
id
a5c05b9f-1603-4c2d-8f11-e90640884891 (old id 2551569)
date added to LUP
2012-06-01 08:50:08
date last changed
2017-10-29 03:45:55
@article{a5c05b9f-1603-4c2d-8f11-e90640884891,
  abstract     = {In epidemiological studies, Helicobacter pylori infection is usually detected by enzyme-linked immunosorbent assay (ELISA). However, infection can spontaneously clear from the mucosa during the progression of atrophy and could lead to substantial under-detection of infection and underestimation of its effect on gastric cancer (GC) risk. Antibodies detected by western blot are known to persist longer after the loss of the infection. In a nested case-control study from the Eurogast-EPIC cohort, including 88 noncardia GC cases and 338 controls, we assessed the association between noncardia GC and H. pylori infection comparing antibodies detected by western blot (HELICOBLOT2.1) to those detected by ELISA (Pyloriset EIA-GIII((R))). By immunoblot, 82 cases (93.2%) were H. pylori positive, 10 of these cases (11.4%) were negative by ELISA and only 6 cases (6.8%) were negative by both ELISA and immunoblot. Multivariable odds ratio (OR) for noncardia GC comparing immunoglobulin G positive versus negative by ELISA was 6.8 [95% confidence interval (CI) 3.0-15.1], and by immunoblot, the OR was 21.4 (95% CI 7.1-64.4). Using a western blot assay, nearly all noncardia GC were classified as H. pylori positive and the OR was more than threefold higher than the OR assessed by ELISA, supporting the hypothesis that H. pylori infection is a necessary condition for noncardia GC.},
  author       = {Gonzalez, C. A. and Megraud, F. and Buissonniere, A. and Lujan Barroso, L. and Agudo, A. and Duell, E. J. and Boutron-Ruault, M. C. and Clavel-Chapelon, F. and Palli, D. and Krogh, V. and Mattiello, A. and Tumino, R. and Sacerdote, C. and Quiros, J. R. and Sanchez-Cantalejo, E. and Navarro, C. and Barricarte, A. and Dorronsoro, M. and Khaw, K. -T. and Wareham, N. and Allen, N. E. and Tsilidis, K. K. and Bueno-de-Mesquita, H. Bas and Jeurnink, S. M. and Numans, M. E. and Peeters, P. H. M. and Lagiou, P. and Valanou, E. and Trichopoulou, A. and Kaaks, R. and Lukanova-McGregor, A. and Bergman, M. M. and Boeing, H. and Manjer, Jonas and Lindkvist, B. and Stenling, R. and Hallmans, G. and Mortensen, L. M. and Overvad, K. and Olsen, A. and Tjonneland, A. and Bakken, K. and Dumeaux, V. and Lund, E. and Jenab, M. and Romieu, I. and Michaud, D. and Mouw, T. and Carneiro, F. and Fenge, C. and Riboli, E.},
  issn         = {1569-8041},
  keyword      = {Helicobacter pylori,noncardia gastric cancer,prospective study,western blot},
  language     = {eng},
  number       = {5},
  pages        = {1320--1324},
  publisher    = {Oxford University Press},
  series       = {Annals of Oncology},
  title        = {Helicobacter pylori infection assessed by ELISA and by immunoblot and noncardia gastric cancer risk in a prospective study: the Eurgast-EPIC project},
  url          = {http://dx.doi.org/10.1093/annonc/mdr384},
  volume       = {23},
  year         = {2012},
}