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Mechanistic Basis of Branch-Site Selection in Filamentous Bacteria

Richards, David M.; Hempel, Antje M.; Flärdh, Klas LU ; Buttner, Mark J. and Howard, Martin (2012) In PLoS Computational Biology 8(3).
Abstract
Many filamentous organisms, such as fungi, grow by tip-extension and by forming new branches behind the tips. A similar growth mode occurs in filamentous bacteria, including the genus Streptomyces, although here our mechanistic understanding has been very limited. The Streptomyces protein DivIVA is a critical determinant of hyphal growth and localizes in foci at hyphal tips and sites of future branch development. However, how such foci form was previously unknown. Here, we show experimentally that DivIVA focus-formation involves a novel mechanism in which new DivIVA foci break off from existing tip-foci, bypassing the need for initial nucleation or de novo branch-site selection. We develop a mathematical model for DivIVA-dependent growth... (More)
Many filamentous organisms, such as fungi, grow by tip-extension and by forming new branches behind the tips. A similar growth mode occurs in filamentous bacteria, including the genus Streptomyces, although here our mechanistic understanding has been very limited. The Streptomyces protein DivIVA is a critical determinant of hyphal growth and localizes in foci at hyphal tips and sites of future branch development. However, how such foci form was previously unknown. Here, we show experimentally that DivIVA focus-formation involves a novel mechanism in which new DivIVA foci break off from existing tip-foci, bypassing the need for initial nucleation or de novo branch-site selection. We develop a mathematical model for DivIVA-dependent growth and branching, involving DivIVA focus-formation by tip-focus splitting, focus growth, and the initiation of new branches at a critical focus size. We quantitatively fit our model to the experimentally-measured tip-to-branch and branch-to-branch length distributions. The model predicts a particular bimodal tip-to-branch distribution results from tip-focus splitting, a prediction we confirm experimentally. Our work provides mechanistic understanding of a novel mode of hyphal growth regulation that may be widely employed. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
PLoS Computational Biology
volume
8
issue
3
publisher
Public Library of Science
external identifiers
  • wos:000302244000031
  • scopus:84861144319
ISSN
1553-7358
DOI
10.1371/journal.pcbi.1002423
language
English
LU publication?
yes
id
b1d4608a-bd3d-482b-ba22-ee29116aa056 (old id 2591486)
date added to LUP
2012-05-29 13:55:37
date last changed
2017-08-13 03:03:34
@article{b1d4608a-bd3d-482b-ba22-ee29116aa056,
  abstract     = {Many filamentous organisms, such as fungi, grow by tip-extension and by forming new branches behind the tips. A similar growth mode occurs in filamentous bacteria, including the genus Streptomyces, although here our mechanistic understanding has been very limited. The Streptomyces protein DivIVA is a critical determinant of hyphal growth and localizes in foci at hyphal tips and sites of future branch development. However, how such foci form was previously unknown. Here, we show experimentally that DivIVA focus-formation involves a novel mechanism in which new DivIVA foci break off from existing tip-foci, bypassing the need for initial nucleation or de novo branch-site selection. We develop a mathematical model for DivIVA-dependent growth and branching, involving DivIVA focus-formation by tip-focus splitting, focus growth, and the initiation of new branches at a critical focus size. We quantitatively fit our model to the experimentally-measured tip-to-branch and branch-to-branch length distributions. The model predicts a particular bimodal tip-to-branch distribution results from tip-focus splitting, a prediction we confirm experimentally. Our work provides mechanistic understanding of a novel mode of hyphal growth regulation that may be widely employed.},
  author       = {Richards, David M. and Hempel, Antje M. and Flärdh, Klas and Buttner, Mark J. and Howard, Martin},
  issn         = {1553-7358},
  language     = {eng},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PLoS Computational Biology},
  title        = {Mechanistic Basis of Branch-Site Selection in Filamentous Bacteria},
  url          = {http://dx.doi.org/10.1371/journal.pcbi.1002423},
  volume       = {8},
  year         = {2012},
}