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Imaging meets cytometry : Analyzing heterogeneous functional microscopic data from living cell populations

Draper, Matthew ; Willems, Mara ; Malahe, Reshwan K. ; Hamilton, Alexander LU and Tarasov, Andrei I. (2021) In Journal of Imaging 7(1).
Abstract

Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships... (More)

Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships between islet cell populations. We therefore believe that the functional cytometric approach can be used for routine quantitative profiling of the tissue for drug screening or pathological testing.

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Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antidiabetic drugs, Baseline correction, Cell profiling, Fluorescence, Time-lapse imaging
in
Journal of Imaging
volume
7
issue
1
article number
9
publisher
MDPI AG
external identifiers
  • pmid:34460580
  • scopus:85103601776
ISSN
2313-433X
DOI
10.3390/jimaging7010009
language
English
LU publication?
yes
id
25969c3d-b15d-48b6-bda7-28ac0c780970
date added to LUP
2021-04-13 13:42:47
date last changed
2024-07-13 12:12:08
@article{25969c3d-b15d-48b6-bda7-28ac0c780970,
  abstract     = {{<p>Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships between islet cell populations. We therefore believe that the functional cytometric approach can be used for routine quantitative profiling of the tissue for drug screening or pathological testing.</p>}},
  author       = {{Draper, Matthew and Willems, Mara and Malahe, Reshwan K. and Hamilton, Alexander and Tarasov, Andrei I.}},
  issn         = {{2313-433X}},
  keywords     = {{Antidiabetic drugs; Baseline correction; Cell profiling; Fluorescence; Time-lapse imaging}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Journal of Imaging}},
  title        = {{Imaging meets cytometry : Analyzing heterogeneous functional microscopic data from living cell populations}},
  url          = {{http://dx.doi.org/10.3390/jimaging7010009}},
  doi          = {{10.3390/jimaging7010009}},
  volume       = {{7}},
  year         = {{2021}},
}