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3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices

Kajtez, Janko LU orcid ; Buchmann, Sebastian ; Vasudevan, Shashank ; Birtele, Marcella LU orcid ; Rocchetti, Stefano ; Pless, Christian Jonathan ; Heiskanen, Arto LU ; Barker, Roger A. LU ; Martínez-Serrano, Alberto and Parmar, Malin LU orcid , et al. (2020) In Advanced Science 7(16).
Abstract

Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by the lack of a simple fabrication approach for the creation of intricate device architectures with high-aspect ratio features. Here, a hybrid additive manufacturing approach is presented for the fabrication of open-well compartmentalized neural devices that provides larger freedom of device design, removes the need for manual postprocessing, and allows an increase in the biocompatibility of the system. Suitability of the method for multimaterial integration allows to tailor the device architecture for the long-term maintenance of healthy human stem-cell derived neurons and... (More)

Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by the lack of a simple fabrication approach for the creation of intricate device architectures with high-aspect ratio features. Here, a hybrid additive manufacturing approach is presented for the fabrication of open-well compartmentalized neural devices that provides larger freedom of device design, removes the need for manual postprocessing, and allows an increase in the biocompatibility of the system. Suitability of the method for multimaterial integration allows to tailor the device architecture for the long-term maintenance of healthy human stem-cell derived neurons and astrocytes, spanning at least 40 days. Leveraging fast-prototyping capabilities at both micro and macroscale, a proof-of-principle human in vitro model of the nigrostriatal pathway is created. By presenting a route for novel materials and unique architectures in microfluidic systems, the method provides new possibilities in biological research beyond neuroscience applications.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3D printing, compartmentalized devices, fast prototyping, human neural stem cells, neurite guidance, nigrostriatal pathway, soft lithography
in
Advanced Science
volume
7
issue
16
article number
2001150
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85086403036
  • pmid:32832365
ISSN
2198-3844
DOI
10.1002/advs.202001150
language
English
LU publication?
yes
id
25d4bdda-1994-4c87-92d6-2da3ae622a5c
date added to LUP
2020-06-30 16:14:51
date last changed
2024-06-13 19:03:32
@article{25d4bdda-1994-4c87-92d6-2da3ae622a5c,
  abstract     = {{<p>Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by the lack of a simple fabrication approach for the creation of intricate device architectures with high-aspect ratio features. Here, a hybrid additive manufacturing approach is presented for the fabrication of open-well compartmentalized neural devices that provides larger freedom of device design, removes the need for manual postprocessing, and allows an increase in the biocompatibility of the system. Suitability of the method for multimaterial integration allows to tailor the device architecture for the long-term maintenance of healthy human stem-cell derived neurons and astrocytes, spanning at least 40 days. Leveraging fast-prototyping capabilities at both micro and macroscale, a proof-of-principle human in vitro model of the nigrostriatal pathway is created. By presenting a route for novel materials and unique architectures in microfluidic systems, the method provides new possibilities in biological research beyond neuroscience applications.</p>}},
  author       = {{Kajtez, Janko and Buchmann, Sebastian and Vasudevan, Shashank and Birtele, Marcella and Rocchetti, Stefano and Pless, Christian Jonathan and Heiskanen, Arto and Barker, Roger A. and Martínez-Serrano, Alberto and Parmar, Malin and Lind, Johan Ulrik and Emnéus, Jenny}},
  issn         = {{2198-3844}},
  keywords     = {{3D printing; compartmentalized devices; fast prototyping; human neural stem cells; neurite guidance; nigrostriatal pathway; soft lithography}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{16}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Advanced Science}},
  title        = {{3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices}},
  url          = {{http://dx.doi.org/10.1002/advs.202001150}},
  doi          = {{10.1002/advs.202001150}},
  volume       = {{7}},
  year         = {{2020}},
}