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Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici

Dishisha, Tarek LU ; Alvarez Aliaga, Teresa LU and Hatti-Kaul, Rajni LU (2012) In Bioresource Technology 118C. p.553-562
Abstract
Propionicacid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionicacid from 40 g.L-1 glycerol were 0.29 g.L-1.h-1, 0.74 mol.mol-1 and 20.09 g.L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amount of cells than PEI-Luffa and produced 20, 28 and 35 g.L-1propionicacid from 40, 65 and 85 g.L-1 glycerol, respectively (0.61 molPA.molGly-1). The corresponding production rates were 0.86, 0.43 and 0.35 g.L-1.h-1, which are the highest reported from glycerol via batch or... (More)
Propionicacid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionicacid from 40 g.L-1 glycerol were 0.29 g.L-1.h-1, 0.74 mol.mol-1 and 20.09 g.L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amount of cells than PEI-Luffa and produced 20, 28 and 35 g.L-1propionicacid from 40, 65 and 85 g.L-1 glycerol, respectively (0.61 molPA.molGly-1). The corresponding production rates were 0.86, 0.43 and 0.35 g.L-1.h-1, which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionicacid concentrations. Using a continuous mode of operation at a dilution rate of 0.1 h-1, cell washout was observed in the bioreactor with free cells; however, propionicacid productivity, yield and concentration were 1.4 g.L-1.h-1, 0.86 molPA.molGly-1, and 14.5 g.L-1, respectively, using immobilized cells in the PEI-Poraver bioreactor. The choice of the immobilization matrix can thus significantly influence the fermentation efficiency and -profile. The bioreactor using cells immobilized on PEI-Poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on PEI-Luffa. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fermentation, Platform chemical, Organic acid, Cell adsorption, Polyethylenimine
in
Bioresource Technology
volume
118C
pages
553 - 562
publisher
Elsevier
external identifiers
  • wos:000307259600074
  • scopus:84862653546
ISSN
1873-2976
DOI
10.1016/j.biortech.2012.05.079
language
English
LU publication?
yes
id
936cae74-ff48-4141-97d1-76246f2a5c48 (old id 2628888)
date added to LUP
2012-06-27 11:24:04
date last changed
2017-11-12 03:46:54
@article{936cae74-ff48-4141-97d1-76246f2a5c48,
  abstract     = {Propionicacid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionicacid from 40 g.L-1 glycerol were 0.29 g.L-1.h-1, 0.74 mol.mol-1 and 20.09 g.L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amount of cells than PEI-Luffa and produced 20, 28 and 35 g.L-1propionicacid from 40, 65 and 85 g.L-1 glycerol, respectively (0.61 molPA.molGly-1). The corresponding production rates were 0.86, 0.43 and 0.35 g.L-1.h-1, which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionicacid concentrations. Using a continuous mode of operation at a dilution rate of 0.1 h-1, cell washout was observed in the bioreactor with free cells; however, propionicacid productivity, yield and concentration were 1.4 g.L-1.h-1, 0.86 molPA.molGly-1, and 14.5 g.L-1, respectively, using immobilized cells in the PEI-Poraver bioreactor. The choice of the immobilization matrix can thus significantly influence the fermentation efficiency and -profile. The bioreactor using cells immobilized on PEI-Poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on PEI-Luffa.},
  author       = {Dishisha, Tarek and Alvarez Aliaga, Teresa and Hatti-Kaul, Rajni},
  issn         = {1873-2976},
  keyword      = {Fermentation,Platform chemical,Organic acid,Cell adsorption,Polyethylenimine},
  language     = {eng},
  pages        = {553--562},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici},
  url          = {http://dx.doi.org/10.1016/j.biortech.2012.05.079},
  volume       = {118C},
  year         = {2012},
}