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NMR, cloud-point measurements and enzymatic depolymerization: Complementary tools to investigate substituent patterns in modified celluloses

Fitzpatrick, Fiona ; Schagerlöf, Herje LU ; Andersson, Thomas ; Richardson, Sara LU ; Tjerneld, Folke LU ; Wahlund, Karl-Gustav LU and Wittgren, Bengt (2006) In Biomacromolecules 7(10). p.2909-2917
Abstract
The substituent patterns of some chemically modified celluloses were characterized as a function of their size distribution, using size-exclusion chromatography coupled to both nuclear magnetic resonance spectroscopy (NMR) and cloud-point measurements. Intact and enzymatically hydrolyzed methyl cellulose (MC) was fractionated according to size, and the level of substitution of the fractions was measured off-line using NMR. Clouding behavior was also measured as a function of size. Clear differences between hydrolyzed and nonhydrolyzed samples were observed using both techniques. For samples that had been selectively hydrolyzed using cellulose-degrading enzymes, NMR data showed a direct link between the degree of degradation and the level... (More)
The substituent patterns of some chemically modified celluloses were characterized as a function of their size distribution, using size-exclusion chromatography coupled to both nuclear magnetic resonance spectroscopy (NMR) and cloud-point measurements. Intact and enzymatically hydrolyzed methyl cellulose (MC) was fractionated according to size, and the level of substitution of the fractions was measured off-line using NMR. Clouding behavior was also measured as a function of size. Clear differences between hydrolyzed and nonhydrolyzed samples were observed using both techniques. For samples that had been selectively hydrolyzed using cellulose-degrading enzymes, NMR data showed a direct link between the degree of degradation and the level of substitution. Differences in the clouding behavior highlighted changes in substituent levels and substituent patterns across the size distribution. The techniques gave valuable and somewhat complementary information on the substituent distributions of the samples before and after enzymatic hydrolysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
7
issue
10
pages
2909 - 2917
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000241106600029
  • scopus:33750375024
  • pmid:17025369
ISSN
1526-4602
DOI
10.1021/bm060281o
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
id
26ccf67d-70d4-4cde-a479-cca641f868d7 (old id 388583)
date added to LUP
2016-04-01 11:52:32
date last changed
2020-08-12 03:12:45
@article{26ccf67d-70d4-4cde-a479-cca641f868d7,
  abstract     = {The substituent patterns of some chemically modified celluloses were characterized as a function of their size distribution, using size-exclusion chromatography coupled to both nuclear magnetic resonance spectroscopy (NMR) and cloud-point measurements. Intact and enzymatically hydrolyzed methyl cellulose (MC) was fractionated according to size, and the level of substitution of the fractions was measured off-line using NMR. Clouding behavior was also measured as a function of size. Clear differences between hydrolyzed and nonhydrolyzed samples were observed using both techniques. For samples that had been selectively hydrolyzed using cellulose-degrading enzymes, NMR data showed a direct link between the degree of degradation and the level of substitution. Differences in the clouding behavior highlighted changes in substituent levels and substituent patterns across the size distribution. The techniques gave valuable and somewhat complementary information on the substituent distributions of the samples before and after enzymatic hydrolysis.},
  author       = {Fitzpatrick, Fiona and Schagerlöf, Herje and Andersson, Thomas and Richardson, Sara and Tjerneld, Folke and Wahlund, Karl-Gustav and Wittgren, Bengt},
  issn         = {1526-4602},
  language     = {eng},
  number       = {10},
  pages        = {2909--2917},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Biomacromolecules},
  title        = {NMR, cloud-point measurements and enzymatic depolymerization: Complementary tools to investigate substituent patterns in modified celluloses},
  url          = {http://dx.doi.org/10.1021/bm060281o},
  doi          = {10.1021/bm060281o},
  volume       = {7},
  year         = {2006},
}