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The major glucagon-like peptide-1 metabolite, GLP-1-(9-36)-amide, does not affect glucose or insulin levels in mice

Rolin, B; Deacon, CF; Carr, RD and Ahrén, Bo LU (2004) In European Journal of Pharmacology 494(2-3). p.283-288
Abstract
Glucagon-like peptide-1 (GLP-1), a future treatment for type 2 diabetes, is efficiently degraded by the enzyme dipeptidyl peptidase IV (DPP IV), yielding the major metabolite GLP-1-(9-36)-amide. In this study, we examined the potential glucose lowering effect of GLP-1(9-36)-amide in mice and found that GLP-1-(9-36)-amide (3 and 10 nmol/kg) did not affect insulin secretion or glucose elimination when administered intravenously together with glucose (1 g/kg). This was observed both in normal mice and in transgenic mice having a complete disruption of the signalling from the GLP-1 receptor. Furthermore, after blocking insulin secretion, using diazoxide (25 mg/kg), no effect on insulin-independent glucose disposal of GLP-1-(9-36)-amide was... (More)
Glucagon-like peptide-1 (GLP-1), a future treatment for type 2 diabetes, is efficiently degraded by the enzyme dipeptidyl peptidase IV (DPP IV), yielding the major metabolite GLP-1-(9-36)-amide. In this study, we examined the potential glucose lowering effect of GLP-1(9-36)-amide in mice and found that GLP-1-(9-36)-amide (3 and 10 nmol/kg) did not affect insulin secretion or glucose elimination when administered intravenously together with glucose (1 g/kg). This was observed both in normal mice and in transgenic mice having a complete disruption of the signalling from the GLP-1 receptor. Furthermore, after blocking insulin secretion, using diazoxide (25 mg/kg), no effect on insulin-independent glucose disposal of GLP-1-(9-36)-amide was observed. Therefore, GLP-1-(9-36)-amide does not affect glucose disposal in mice either in the presence or absence of intact GLP-1-receptors or in the presence or absence of stimulated insulin levels. This suggests that the GLP-1 metabolite is not involved in the regulation of glucose homeostasis. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transgenic animal, glucagon like peptide 1, dipeptidyl peptidase IV, model, diabetes
in
European Journal of Pharmacology
volume
494
issue
2-3
pages
283 - 288
publisher
Elsevier
external identifiers
  • wos:000222398600025
  • pmid:15212985
  • scopus:2942755813
ISSN
1879-0712
DOI
10.1016/j.ejphar.2004.05.013
language
English
LU publication?
yes
id
b645fa64-83dd-4844-a267-1c38e7cba147 (old id 273580)
date added to LUP
2007-10-31 09:13:45
date last changed
2017-12-10 03:39:07
@article{b645fa64-83dd-4844-a267-1c38e7cba147,
  abstract     = {Glucagon-like peptide-1 (GLP-1), a future treatment for type 2 diabetes, is efficiently degraded by the enzyme dipeptidyl peptidase IV (DPP IV), yielding the major metabolite GLP-1-(9-36)-amide. In this study, we examined the potential glucose lowering effect of GLP-1(9-36)-amide in mice and found that GLP-1-(9-36)-amide (3 and 10 nmol/kg) did not affect insulin secretion or glucose elimination when administered intravenously together with glucose (1 g/kg). This was observed both in normal mice and in transgenic mice having a complete disruption of the signalling from the GLP-1 receptor. Furthermore, after blocking insulin secretion, using diazoxide (25 mg/kg), no effect on insulin-independent glucose disposal of GLP-1-(9-36)-amide was observed. Therefore, GLP-1-(9-36)-amide does not affect glucose disposal in mice either in the presence or absence of intact GLP-1-receptors or in the presence or absence of stimulated insulin levels. This suggests that the GLP-1 metabolite is not involved in the regulation of glucose homeostasis. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Rolin, B and Deacon, CF and Carr, RD and Ahrén, Bo},
  issn         = {1879-0712},
  keyword      = {transgenic animal,glucagon like peptide 1,dipeptidyl peptidase IV,model,diabetes},
  language     = {eng},
  number       = {2-3},
  pages        = {283--288},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {The major glucagon-like peptide-1 metabolite, GLP-1-(9-36)-amide, does not affect glucose or insulin levels in mice},
  url          = {http://dx.doi.org/10.1016/j.ejphar.2004.05.013},
  volume       = {494},
  year         = {2004},
}