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Effects of a high-fat diet during pregnancy and lactation are modulated by E. coli in rat offspring

Fak, Frida; Linninge, Caroline LU ; Ahrné, Siv LU ; Molin, Göran LU and Weström, Björn LU (2012) In International Journal of Obesity 36(5). p.744-751
Abstract
Objective: Microbial manipulations in early life can affect gut development and inflammatory status of the neonate. The maternal diet during pregnancy and lactation also influences the health of the offspring, but the impact of maternal high-fat (HF) feeding along with modulations of the gut microbiota on body weight, fat deposition and gut function in the offspring has been poorly studied. Methods: Rat dams were given access to either an HF or a standard low-fat diet during the last 2 weeks of pregnancy and during lactation and effects on body weight and gastrointestinal function were investigated in the 14-day-old offspring. To elucidate whether bacterial administration to the dam could modulate any effects of the diets in the rat pups,... (More)
Objective: Microbial manipulations in early life can affect gut development and inflammatory status of the neonate. The maternal diet during pregnancy and lactation also influences the health of the offspring, but the impact of maternal high-fat (HF) feeding along with modulations of the gut microbiota on body weight, fat deposition and gut function in the offspring has been poorly studied. Methods: Rat dams were given access to either an HF or a standard low-fat diet during the last 2 weeks of pregnancy and during lactation and effects on body weight and gastrointestinal function were investigated in the 14-day-old offspring. To elucidate whether bacterial administration to the dam could modulate any effects of the diets in the rat pups, another group of dams were given Escherichia coli in their drinking water. Results: Maternal HF feeding resulted in increased body and fat pad weights in the offspring, along with increased levels of the acute-phase protein, haptoglobin and decreased protein content and disaccharidase activities in the small intestine. The addition of E. coli further accentuated these responses in the young rats, which, in addition to higher body weights and increased fat deposition, also showed an increased intestinal permeability and elevated levels of haptoglobin. Conclusions: The present study demonstrates for the first time how bacterial administration to the maternal diet during the neonatal period can affect body weight and fat deposition in the offspring. The results point to a mechanistic link between the gut microbiota, increased intestinal permeability and metabolic endotoxemia, which appear to have led to increased adiposity in the young rats. International Journal of Obesity (2012) 36, 744-751; doi:10.1038/ijo.2011.118; published online 5 July 2011 (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bacteria, gastrointestinal, rat, body weight, fat, permeability
in
International Journal of Obesity
volume
36
issue
5
pages
744 - 751
publisher
Nature Publishing Group
external identifiers
  • wos:000303771000015
  • scopus:84860778010
ISSN
1476-5497
DOI
10.1038/ijo.2011.118
language
English
LU publication?
yes
id
52cff112-6e2f-4317-80c2-9e346ba453d5 (old id 2826746)
date added to LUP
2012-06-20 14:06:31
date last changed
2017-08-20 03:25:44
@article{52cff112-6e2f-4317-80c2-9e346ba453d5,
  abstract     = {Objective: Microbial manipulations in early life can affect gut development and inflammatory status of the neonate. The maternal diet during pregnancy and lactation also influences the health of the offspring, but the impact of maternal high-fat (HF) feeding along with modulations of the gut microbiota on body weight, fat deposition and gut function in the offspring has been poorly studied. Methods: Rat dams were given access to either an HF or a standard low-fat diet during the last 2 weeks of pregnancy and during lactation and effects on body weight and gastrointestinal function were investigated in the 14-day-old offspring. To elucidate whether bacterial administration to the dam could modulate any effects of the diets in the rat pups, another group of dams were given Escherichia coli in their drinking water. Results: Maternal HF feeding resulted in increased body and fat pad weights in the offspring, along with increased levels of the acute-phase protein, haptoglobin and decreased protein content and disaccharidase activities in the small intestine. The addition of E. coli further accentuated these responses in the young rats, which, in addition to higher body weights and increased fat deposition, also showed an increased intestinal permeability and elevated levels of haptoglobin. Conclusions: The present study demonstrates for the first time how bacterial administration to the maternal diet during the neonatal period can affect body weight and fat deposition in the offspring. The results point to a mechanistic link between the gut microbiota, increased intestinal permeability and metabolic endotoxemia, which appear to have led to increased adiposity in the young rats. International Journal of Obesity (2012) 36, 744-751; doi:10.1038/ijo.2011.118; published online 5 July 2011},
  author       = {Fak, Frida and Linninge, Caroline and Ahrné, Siv and Molin, Göran and Weström, Björn},
  issn         = {1476-5497},
  keyword      = {bacteria,gastrointestinal,rat,body weight,fat,permeability},
  language     = {eng},
  number       = {5},
  pages        = {744--751},
  publisher    = {Nature Publishing Group},
  series       = {International Journal of Obesity},
  title        = {Effects of a high-fat diet during pregnancy and lactation are modulated by E. coli in rat offspring},
  url          = {http://dx.doi.org/10.1038/ijo.2011.118},
  volume       = {36},
  year         = {2012},
}