Endocardial expression and functional characterization of endothelin-1
(2001) In Molecular and Cellular Biochemistry 224(1-2). p.151-158- Abstract
- Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established... (More)
- Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established as the conditioned media obtained from cultured porcine endocardial endothelial cells induced a slowly developing, strong and long-lasting contraction of circular rat aortic segments, with similar characteristics to that obtained with exogenous ET-1. Furthermore, the selective endothelin-A receptor antagonist, FR 139317, blocked the conditioned media induced contractions. Our results suggest that endocardial endothelial cells express and release biologically active ET-1 which could play a pivotal role in the regulation of myocardial contractility as well as a circulatory peptide may further act in other peripheral target organs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121355
- author
- Saetrum Opgaard, Ole ; Adner, Mikael LU ; Peters, Theodorus H.F. ; Xu, Cang-Bao LU ; Stavenow, Lars ; Gulbenkian, Sergio ; Erlinge, David LU ; Edvinsson, Lars LU and Sharma, Hari S.
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- endothelin1, endocardial endothelial cells, immunofluorescence, in vitro pharmacology, FR139317
- in
- Molecular and Cellular Biochemistry
- volume
- 224
- issue
- 1-2
- pages
- 151 - 158
- publisher
- Springer
- external identifiers
-
- pmid:11693192
- scopus:0034800119
- ISSN
- 0300-8177
- DOI
- 10.1023/A:1011952504093
- language
- English
- LU publication?
- yes
- id
- 2838ae92-45d8-42b0-a7b3-723d8fdf050a (old id 1121355)
- date added to LUP
- 2016-04-01 16:51:02
- date last changed
- 2024-01-11 16:03:07
@article{2838ae92-45d8-42b0-a7b3-723d8fdf050a, abstract = {{Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established as the conditioned media obtained from cultured porcine endocardial endothelial cells induced a slowly developing, strong and long-lasting contraction of circular rat aortic segments, with similar characteristics to that obtained with exogenous ET-1. Furthermore, the selective endothelin-A receptor antagonist, FR 139317, blocked the conditioned media induced contractions. Our results suggest that endocardial endothelial cells express and release biologically active ET-1 which could play a pivotal role in the regulation of myocardial contractility as well as a circulatory peptide may further act in other peripheral target organs.}}, author = {{Saetrum Opgaard, Ole and Adner, Mikael and Peters, Theodorus H.F. and Xu, Cang-Bao and Stavenow, Lars and Gulbenkian, Sergio and Erlinge, David and Edvinsson, Lars and Sharma, Hari S.}}, issn = {{0300-8177}}, keywords = {{endothelin1; endocardial endothelial cells; immunofluorescence; in vitro pharmacology; FR139317}}, language = {{eng}}, number = {{1-2}}, pages = {{151--158}}, publisher = {{Springer}}, series = {{Molecular and Cellular Biochemistry}}, title = {{Endocardial expression and functional characterization of endothelin-1}}, url = {{http://dx.doi.org/10.1023/A:1011952504093}}, doi = {{10.1023/A:1011952504093}}, volume = {{224}}, year = {{2001}}, }