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Edible proteins from coconut milk press cake; one step alkaline extraction and characterization by electrophoresis and mass spectrometry

Chambale, Borges LU ; Bergenståhl, Björn LU and Dejmek, Petr LU (2012) In Food Research International 47(2). p.146-151
Abstract
Coconut milk press cake, the spent endosperm left over after the extraction of coconut milk, was utilized to extract additional protein under alkaline conditions. The maximum protein yield was observed at 50 degrees C and pH 11 and corresponded to total endosperm protein yield of 71%, favourably comparable to yields of more complex extractions. The extract was characterized by amino acid analysis and 1D SDS-PAGE, 2D electrophoresis and MS MS spectroscopy. Coconut milk and insoluble milk sediment from the same individual nuts were also analyzed. The alkaline extract reproducibly differed from the other fractions and from those reported by others in amino acid content as seen in Principal Component Analysis, and had in particular higher... (More)
Coconut milk press cake, the spent endosperm left over after the extraction of coconut milk, was utilized to extract additional protein under alkaline conditions. The maximum protein yield was observed at 50 degrees C and pH 11 and corresponded to total endosperm protein yield of 71%, favourably comparable to yields of more complex extractions. The extract was characterized by amino acid analysis and 1D SDS-PAGE, 2D electrophoresis and MS MS spectroscopy. Coconut milk and insoluble milk sediment from the same individual nuts were also analyzed. The alkaline extract reproducibly differed from the other fractions and from those reported by others in amino acid content as seen in Principal Component Analysis, and had in particular higher threonine content The peptide found in the alkaline extract but with no counterpart in coconut milk or its sediment showed IP (approximate to 5) and mass (approximate to 40 kDa) and matched partially glutelin from oil palm. (c) 2011 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alkaline extraction, Electrophoresis, Mass, Coconut proteins, spectrometry
in
Food Research International
volume
47
issue
2
pages
146 - 151
publisher
Elsevier
external identifiers
  • wos:000305772400005
  • scopus:84861731487
ISSN
0963-9969
DOI
10.1016/j.foodres.2011.04.036
language
English
LU publication?
yes
id
fb424558-eec1-4675-9771-9b3465bb29cc (old id 2863758)
date added to LUP
2012-07-24 15:26:46
date last changed
2017-09-10 03:21:00
@article{fb424558-eec1-4675-9771-9b3465bb29cc,
  abstract     = {Coconut milk press cake, the spent endosperm left over after the extraction of coconut milk, was utilized to extract additional protein under alkaline conditions. The maximum protein yield was observed at 50 degrees C and pH 11 and corresponded to total endosperm protein yield of 71%, favourably comparable to yields of more complex extractions. The extract was characterized by amino acid analysis and 1D SDS-PAGE, 2D electrophoresis and MS MS spectroscopy. Coconut milk and insoluble milk sediment from the same individual nuts were also analyzed. The alkaline extract reproducibly differed from the other fractions and from those reported by others in amino acid content as seen in Principal Component Analysis, and had in particular higher threonine content The peptide found in the alkaline extract but with no counterpart in coconut milk or its sediment showed IP (approximate to 5) and mass (approximate to 40 kDa) and matched partially glutelin from oil palm. (c) 2011 Elsevier Ltd. All rights reserved.},
  author       = {Chambale, Borges and Bergenståhl, Björn and Dejmek, Petr},
  issn         = {0963-9969},
  keyword      = {Alkaline extraction,Electrophoresis,Mass,Coconut proteins,spectrometry},
  language     = {eng},
  number       = {2},
  pages        = {146--151},
  publisher    = {Elsevier},
  series       = {Food Research International},
  title        = {Edible proteins from coconut milk press cake; one step alkaline extraction and characterization by electrophoresis and mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.foodres.2011.04.036},
  volume       = {47},
  year         = {2012},
}