Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - A study of spouses of pregnant women in three geographical regions
(2012) In Reproductive Toxicology 33(4). p.577-583- Abstract
- Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from... (More)
- Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found. (C) 2012 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2891139
- author
- Specht, Ina Olmer ; Hougaard, Karin S. ; Spano, Marcello ; Bizzaro, Davide ; Manicardi, Gian Carlo ; Lindh, Christian LU ; Toft, Gunnar ; Jönsson, Bo A LU ; Giwercman, Aleksander LU and Bonde, Jens Peter E.
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Perfluorinated compounds, PFOS, PFOA, DNA fragmentation index, Sperm, chromatin structure assay, TUNEL assay, Fas receptor, Bcl-xL
- in
- Reproductive Toxicology
- volume
- 33
- issue
- 4
- pages
- 577 - 583
- publisher
- Elsevier
- external identifiers
-
- wos:000305303100017
- scopus:84861532194
- pmid:22449571
- ISSN
- 1873-1708
- DOI
- 10.1016/j.reprotox.2012.02.008
- language
- English
- LU publication?
- yes
- id
- b028baba-c046-4281-ba31-1959388c1f0e (old id 2891139)
- date added to LUP
- 2016-04-01 14:04:05
- date last changed
- 2022-04-22 01:06:18
@article{b028baba-c046-4281-ba31-1959388c1f0e, abstract = {{Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found. (C) 2012 Elsevier Inc. All rights reserved.}}, author = {{Specht, Ina Olmer and Hougaard, Karin S. and Spano, Marcello and Bizzaro, Davide and Manicardi, Gian Carlo and Lindh, Christian and Toft, Gunnar and Jönsson, Bo A and Giwercman, Aleksander and Bonde, Jens Peter E.}}, issn = {{1873-1708}}, keywords = {{Perfluorinated compounds; PFOS; PFOA; DNA fragmentation index; Sperm; chromatin structure assay; TUNEL assay; Fas receptor; Bcl-xL}}, language = {{eng}}, number = {{4}}, pages = {{577--583}}, publisher = {{Elsevier}}, series = {{Reproductive Toxicology}}, title = {{Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - A study of spouses of pregnant women in three geographical regions}}, url = {{http://dx.doi.org/10.1016/j.reprotox.2012.02.008}}, doi = {{10.1016/j.reprotox.2012.02.008}}, volume = {{33}}, year = {{2012}}, }