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Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - A study of spouses of pregnant women in three geographical regions

Specht, Ina Olmer; Hougaard, Karin S.; Spano, Marcello; Bizzaro, Davide; Manicardi, Gian Carlo; Lindh, Christian LU ; Toft, Gunnar; Jönsson, Bo A LU ; Giwercman, Aleksander LU and Bonde, Jens Peter E. (2012) In Reproductive Toxicology 33(4). p.577-583
Abstract
Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from... (More)
Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found. (C) 2012 Elsevier Inc. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Perfluorinated compounds, PFOS, PFOA, DNA fragmentation index, Sperm, chromatin structure assay, TUNEL assay, Fas receptor, Bcl-xL
in
Reproductive Toxicology
volume
33
issue
4
pages
577 - 583
publisher
Elsevier
external identifiers
  • wos:000305303100017
  • scopus:84861532194
ISSN
1873-1708
DOI
10.1016/j.reprotox.2012.02.008
language
English
LU publication?
yes
id
b028baba-c046-4281-ba31-1959388c1f0e (old id 2891139)
date added to LUP
2012-08-01 08:53:56
date last changed
2017-08-27 04:47:37
@article{b028baba-c046-4281-ba31-1959388c1f0e,
  abstract     = {Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found. (C) 2012 Elsevier Inc. All rights reserved.},
  author       = {Specht, Ina Olmer and Hougaard, Karin S. and Spano, Marcello and Bizzaro, Davide and Manicardi, Gian Carlo and Lindh, Christian and Toft, Gunnar and Jönsson, Bo A and Giwercman, Aleksander and Bonde, Jens Peter E.},
  issn         = {1873-1708},
  keyword      = {Perfluorinated compounds,PFOS,PFOA,DNA fragmentation index,Sperm,chromatin structure assay,TUNEL assay,Fas receptor,Bcl-xL},
  language     = {eng},
  number       = {4},
  pages        = {577--583},
  publisher    = {Elsevier},
  series       = {Reproductive Toxicology},
  title        = {Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - A study of spouses of pregnant women in three geographical regions},
  url          = {http://dx.doi.org/10.1016/j.reprotox.2012.02.008},
  volume       = {33},
  year         = {2012},
}