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Identification of a mitochondrial external NADPH dehydrogenase by overexpression in transgenic Nicotiana sylvestris

Michalecka, Agnieszka LU ; Agius, Stephanie C; Moller, Ian M and Rasmusson, Allan LU (2004) In Plant Journal 37(3). p.415-425
Abstract
The plant respiratory chain contains a complex setup of non-energy conserving NAD(P)H dehydrogenases, the physiological consequences of which are highly unclear. An expression construct for the potato (Solanum tuberosum L., cv. Desiree) ndb1 gene, a homologue of bacterial and fungal type II NAD(P)H dehydrogenases, was introduced into Nicotiana sylvestris. Transgenic lines with high transcript and protein levels for St-NDB1 had up to threefold increased activity of external NADPH dehydrogenase in isolated mitochondria as compared to the wild type (WT). In two lines, the external NADPH dehydrogenase activity was instead 10-fold decreased, indicating that the corresponding N. sylvestris gene had been suppressed. Activities of external and... (More)
The plant respiratory chain contains a complex setup of non-energy conserving NAD(P)H dehydrogenases, the physiological consequences of which are highly unclear. An expression construct for the potato (Solanum tuberosum L., cv. Desiree) ndb1 gene, a homologue of bacterial and fungal type II NAD(P)H dehydrogenases, was introduced into Nicotiana sylvestris. Transgenic lines with high transcript and protein levels for St-NDB1 had up to threefold increased activity of external NADPH dehydrogenase in isolated mitochondria as compared to the wild type (WT). In two lines, the external NADPH dehydrogenase activity was instead 10-fold decreased, indicating that the corresponding N. sylvestris gene had been suppressed. Activities of external and internal rotenone-insensitive NADH dehydrogenases were unchanged in the transgenic lines. The results demonstrate that the St-ndb1 encodes an external dehydrogenase specific for NADPH and dependent on calcium for activity. Transgenic lines overexpressing St-ndb1 had specifically increased protein levels for alternative oxidase and uncoupling protein, as compared to the WT and one co-suppressing line. This indicates cross-talk in the expressional control, or metabolic conditions influencing it, for the different categories of energy-dissipating proteins that bypass oxidative phosphorylation. The potential effects of external NADPH oxidation on other cellular processes are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
NAD(P)H dehydrogenase, alternative oxidase, plant mitochondria, respiratory chain, uncoupling protein, transgenic plants
in
Plant Journal
volume
37
issue
3
pages
415 - 425
publisher
Wiley-Blackwell
external identifiers
  • pmid:14731260
  • wos:000188305500009
  • scopus:1042290216
ISSN
1365-313X
DOI
10.1046/j.1365-313X.2003.01970.x
language
English
LU publication?
yes
id
14fa1075-d286-4b99-b282-89c0f0665a27 (old id 289628)
date added to LUP
2007-10-28 14:59:00
date last changed
2017-09-03 04:39:43
@article{14fa1075-d286-4b99-b282-89c0f0665a27,
  abstract     = {The plant respiratory chain contains a complex setup of non-energy conserving NAD(P)H dehydrogenases, the physiological consequences of which are highly unclear. An expression construct for the potato (Solanum tuberosum L., cv. Desiree) ndb1 gene, a homologue of bacterial and fungal type II NAD(P)H dehydrogenases, was introduced into Nicotiana sylvestris. Transgenic lines with high transcript and protein levels for St-NDB1 had up to threefold increased activity of external NADPH dehydrogenase in isolated mitochondria as compared to the wild type (WT). In two lines, the external NADPH dehydrogenase activity was instead 10-fold decreased, indicating that the corresponding N. sylvestris gene had been suppressed. Activities of external and internal rotenone-insensitive NADH dehydrogenases were unchanged in the transgenic lines. The results demonstrate that the St-ndb1 encodes an external dehydrogenase specific for NADPH and dependent on calcium for activity. Transgenic lines overexpressing St-ndb1 had specifically increased protein levels for alternative oxidase and uncoupling protein, as compared to the WT and one co-suppressing line. This indicates cross-talk in the expressional control, or metabolic conditions influencing it, for the different categories of energy-dissipating proteins that bypass oxidative phosphorylation. The potential effects of external NADPH oxidation on other cellular processes are discussed.},
  author       = {Michalecka, Agnieszka and Agius, Stephanie C and Moller, Ian M and Rasmusson, Allan},
  issn         = {1365-313X},
  keyword      = {NAD(P)H dehydrogenase,alternative oxidase,plant mitochondria,respiratory chain,uncoupling protein,transgenic plants},
  language     = {eng},
  number       = {3},
  pages        = {415--425},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {Identification of a mitochondrial external NADPH dehydrogenase by overexpression in transgenic Nicotiana sylvestris},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.2003.01970.x},
  volume       = {37},
  year         = {2004},
}